南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (01): 67-70.

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积雪草苷对增生性瘢痕中转化生长因子-β mRNA及基质金属蛋白酶类表达的影响

张涛;荣新洲;杨荣华;利天增;徐盈斌;   

  1. 广州市第一人民医院烧伤科； 中山大学附属第一医院烧伤科； 中山大学附属第一医院烧伤科 广东 广州 510180； 广东 广州 510180； 广东 广州 510080；
  • 出版日期:2006-01-20 发布日期:2006-01-20
  • 基金资助:
    广州市医药卫生科技项目(2005-YB-027)

Effect of asiaticoside on the expression of transforming growth factor-beta mRNA and matrix metalloproteinases in hypertrophic scars

ZHANG Tao, RONG Xin-zhou, YANG Rong-hua, LI Tian-zeng, XU Ying-bin Department of Burns, Guangzhou First Municipal People’s Hospital, Guangzhou 510180, China; Department of Burns, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China   

  1. 广州市第一人民医院烧伤科； 中山大学附属第一医院烧伤科； 中山大学附属第一医院烧伤科 广东 广州 510180； 广东 广州 510180； 广东 广州 510080；
  • Online:2006-01-20 Published:2006-01-20

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摘要： 目的探讨积雪草苷对人增生性瘢痕中转化生长因子β(TGF-β)mRNA和基质金属蛋白酶及其组织抑制剂(MMPS／ TIMPS)表达的影响。方法临床上收集烧伤后5-8个月经积雪草苷治疗和非积雪草苷治疗的增生性瘢痕标本各9例。采用原位杂交和免疫组织化学方法,分别检测增生性瘢痕中TGF-β1mRNA、TGF-β2mRNA、TGF-β3mRNA及MMP1、 MMP1、TIMP1、TIMP2和Ⅰ、Ⅲ型胶原的表达水平,并利用图象分析方法进行结果分析。结果TGF-βmRNA在增生性瘢痕中主要表达于成纤维细胞胞质,经积雪草苷治疗的增生性瘢痕中TGF-β1mRNA表达明显减少,胞质淡染,统计学上与非积雪草苷治疗组TGF-β1mRNA表达有显著性差异(P<0．01);而TGF-β3mRNA在积雪草苷治疗的增生性瘢痕中表达增多,明显高于非积雪草苷治疗组的TGF-β3mRNA表达(P<0．05)。MMPS／TIMPS在增生性瘢痕中也表达于成纤维细胞胞质,经积雪草苷治疗的增生性瘢痕中TIMP1表达明显减少,与非积雪草苷治疗组TIMP1表达有显著性差异 (P<0．01);而MMP1、MMP2、及TIMP2在上述两组中表达无显著性差异(P>0．05)。Ⅰ、Ⅲ型胶原在增生性瘢痕中表达,经积雪草苷治疗的增生性瘢痕中Ⅰ型胶原排列整齐,含量较少,与非积雪草苷治疗组有显著差异(P<0．05);而Ⅲ型胶原在两组增生性瘢痕中表达无显著性差异(P>0．05)。结论积雪草苷通过降低TGF-β1mRNA表达和增加TGF-β3mRNA的表达,引起TIMP1表达明显减少,从而MMP1／TIMP1对比例增加,达到促进Ⅰ型胶原降解,减轻瘢痕增生的作用。

Abstract: Objective To investigate the effect of asiaticoside on the expressions of transforming growth factor (TGF)- βmR-NA, matrix metalloproteinases (MMPS) and tissue inhibitors of metalloproteinases (TIMPs) in postburn hypertrophic scars. Methods Nine specimens of postburn (5-8 months) hypertrophic scars with asiaticoside treatment and 9 without asiaticoside treatment were collected for testing the expressions of MMPS, TIMPs, type Ⅰ and Ⅲ collagen and TGF-β mRNA by immuno-histochemistry and in situ hybridization methods, followed by image analysis of the results. Results The expressions of TGF-β mRNA and MMPS /TIMPS were all detected in the fibroblast cytoplasm. The expression of TGF-β1 mRNA in asiatico-side-treated scars was significantly lower than that in scars without asiaticoside treatment (P<0.01). In contrast, the expression of TGF-β3 mRNA was significantly higher in asiaticoside group (P<0.05). The expression of TIMP1 in asiaticoside group was significantly lower than that in non-asiaticoside group (P<0.01), and the expression of type I collagen in asiaticoside-treated scars was lower than that in non-asiaticoside-treated specimens (P<0.05), but the expression of MMP1, MMP2 and TIMP2 and type Ⅲ collagen exhibited no significant differences between the two groups (P>0.05). Conclusion Asiaticoside can down-regulate TGF-β1 mRNA and TIMP1 expressions and up-regulate TGF-β3 mRNA expression in postburn hypertrophic scars, and is also capable of decomposing the products of type I collagen, contributing to the reduction of hypertrophic scar formation.

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