南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (01): 22-24.

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瘢痕疙瘩家系外周血淋巴细胞永生化方法探讨

严欣;高建华;宋玫;刘小军;陈阳;   

  1. 南方医科大学南方医院整形外科； 南方医科大学南方医院整形外科 广东 广州 510515； 广东 广州 510515；
  • 出版日期:2006-01-20 发布日期:2006-01-20
  • 基金资助:
    基金项目：广东省名医工程(2004)

Establishment of immortalized lymphoblastoid cell bank of a keloid pedigree

YAN Xin, GAO Jian-hua, SONG Mei, LIU Xiao-jun, CHEN Yang Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院整形外科； 南方医科大学南方医院整形外科 广东 广州 510515； 广东 广州 510515；
  • Online:2006-01-20 Published:2006-01-20

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摘要： 目的探讨建立瘢痕疙瘩家系永生化细胞库的方法,为研究瘢痕疙瘩的发病机制、诊断和治疗提供长期的标本来源.方法采用EB病毒转化技术,分别采用新鲜血法和冻存血法,将瘢痕疙瘩家系外周血B淋巴细胞转化成永生化淋巴母细胞系.结果成功建立了一瘢痕疙瘩家系中27个个体的永生细胞株;新鲜血法较冻存血法可明显提高一次建系成功率.结论EB病毒转化技术可有效保存原来个体完整的基因组,为以后的相关研究提供了长期的DNA资源.新鲜血法明显优于冻存血法,更适用于永生化细胞库的建立.

Abstract: Objective To investigate a method for establishing immortalized lymphoblastoid cell bank of keloid pedigree so as to provide a long-term source of specimens for keloid research. Methods With Epstein-Barr virus transformation, fresh and frozen blood samples collected from all members of the keloid pedigree were used respectively to establish the immortalized lymphoblastoid cell lines of B lymphocytes. Results Twenty-seven immortalized lymphoblastoid cell lines of the keloid pedigree were obtained successfully, and all cell lines survived cryopreservation in liquid nitrogen. Conclusions The immortalized lymphoblastoid cell bank of the keloid pedigree can preserve the whole gene information and provide long-term DNA sources for keloid research. Preparation of the cell lines with fresh blood is more efficient than that with frozen blood.

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