南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (01): 111-113.
涂小丽;刘宏伟;李才良;任肖华;
TU Xiao-li, LIU Hong-wei, LI Cai-liang, REN Xiao-hua Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; Department .of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Stomatology, Guangdong Headquarter Hospital of Chinese People’s Armed Police Forces, Guangzhou 510516, China; Department of Stomatology, 458 Hospital of PLA, Guangzhou 510293, China
摘要: 目的体外分离培养狗的骨髓基质细胞(BMSCs),诱导其向成骨细胞分化,初步鉴定其成骨潜能和生物学特性。方法抽取毕格犬髂骨骨髓体外分离培养获得BMSCs,DMEM、新生牛血清培养基进行原代培养,部分传代细胞以含10 mmol/L地塞米松、50μg/ml抗坏血酸和10 mmol/L β-甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化。倒置相差显微镜进行细胞形态学和细胞生长增殖观察,Von Kossa法染色检测体外矿化结节形成,细胞碱性磷酸酶染色检测BMSCs 的成骨活性。结果体外分离培养的BMSCs经条件培养基诱导后表现出明显的成骨活性,体外矿化(骨样)结节的Von Kossa染色阳性;传代BMSCs碱性磷酸酶染色阳性。结论体外分离培养的BMSCs中含有骨源性前体细胞,传代细胞具有较强的成骨潜能。 更多还原
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