南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (12): 1549-1551.

• • 上一篇    下一篇

HPLC-ELSD法测定苦杏仁中苦杏仁苷含量的研究

吴昭晖1, 游文玮2   

  1. 1. 南方医科大学分析测试中心, 广东, 广州, 510515;
    2. 南方医科大学化学教研室, 广东, 广州, 510515
  • 出版日期:2005-12-20 发布日期:2005-12-20
  • 基金资助:
    收稿日期:2005-2-24。
    作者简介:吴昭晖(1964- ),女,高级工程师.
    通讯作者:游文玮,男,教授,电话:020-61648197,E-mail:youww@fimmu.com.

Determination of amygdalin content in Semen Armeniacae Amarum by high-performance liquid chromatography with evaporative light-scattering detection

WU Zhao-hui1, YOU Wen-wei2   

  1. 1. 南方医科大学分析测试中心, 广东, 广州, 510515;
    2. 南方医科大学化学教研室, 广东, 广州, 510515
  • Online:2005-12-20 Published:2005-12-20

PDF

1029

摘要： 目的 建立高效液相色谱法-蒸发光散射检测器(HPLC-ELSD)测定苦杏仁中苦杏仁苷含量的的分析方法。方法 采用Hypersil-ODS(4.0mm×250mm,5μm)色谱柱;流动相为甲醇-水(30:70),流速0.5ml/min;柱温25℃;利用ELSD检测器检测:漂移管温度98℃,载气流速3.2L/min。结果 苦杏仁苷进样量在1.0~15.1μg范围内,标准曲线呈良好的线性关系,r=0.9999,苦杏仁苷的平均回收率为99.0%,RSD为2.9%(n=5)。结论 本方法简便快速,结果准确可靠,为苦杏仁苷的含量测定提供了一个新方法,可用于苦杏仁药材及含苦杏仁复方制剂的质量控制。

Abstract: High-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD) was employed for determination of amygdalin content in Semen Armeniacae Amarum. The detection was performed with the column of Hypersil-ODS (4.6 mm×250 mm, 5 μm) and column temperature of 25 ℃. The mobile phase was methanol-water (70:30) with flow rate of 0.5 ml/min. Evaporative light-scattering detector was used and the drift tube temperature was set at 98 ℃ with the gas flow rate of 3.2 L/min. A standard curve was generated, which was linear in the range of 1.0-15.1 μg for amygdalin content (r=0.999 9). The average recovery of amygdalin was 99.0% with RSD of 2.9% (n=5). Besides simplicity and rapidness, the method yields accurate and reproducible results and can therefore be used in the quality control of Semen Armeniacae Amarum.

中图分类号: