姜黄素诱导人黑色素瘤A375细胞凋亡以及对c-myc、caspase-3表达的影响

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (12): 1517-1521.

姜黄素诱导人黑色素瘤A375细胞凋亡以及对c-myc、caspase-3表达的影响

邱实¹, 谭升顺¹, 张江安¹, 刘安¹, 袁景奕¹, 饶国洲², 王文勇³

1. 西安交通大学第二医院皮肤科, 陕西, 西安, 710004;
2. 西安交通大学口腔医院医学实验中心, 陕西, 西安, 710004;
3. 第四军医大学基础部病理学教研室, 陕西, 西安, 710032

出版日期:2005-12-20 发布日期:2005-12-20
基金资助:
收稿日期:2005-5-13。
作者简介:邱实(1970- ),男,主治医师,博士研究生,E-mail:qsh0322@163.com.

Apoptosis induced by curcumin and its effect on c-myc and caspase-3 expressions in human melanoma A375 cell line

QIU Shi¹, TAN Sheng-shun¹, ZHANG Jiang-an¹, LIU An¹, YUAN Jing-yi¹, RAO Guo-zhou², WANG Wen-yong³

1. 西安交通大学第二医院皮肤科, 陕西, 西安, 710004;
2. 西安交通大学口腔医院医学实验中心, 陕西, 西安, 710004;
3. 第四军医大学基础部病理学教研室, 陕西, 西安, 710032

Online:2005-12-20 Published:2005-12-20

摘要/Abstract

摘要： 目的探讨姜黄素诱导人黑色素瘤A375细胞凋亡及其对c-myc、Caspase-3表达的影响。方法姜黄素处理A375细胞,MTT法检测细胞的生长活性,应用倒置显微镜和透射电镜进行形态学观察,采用AnnexinV/PI双染法和DAN片段化分析检测细胞凋亡,SABC免疫组化法和原位杂交检测细胞内c-myc、Caspase-3表达水平。结果姜黄素能抑制A375细胞增殖,并呈剂量和时间依赖性。经30 μmol/L姜黄素处理A375细胞48h,细胞呈现凋亡形态学改变;DAN片段化分析可见到明显的DNA梯带形成;流式细胞仪定量检测出20 μmol/L以上浓度姜黄素诱导细胞凋亡的发生率较对照组有显著性差异。c-myc表达水平减少,而Caspase-3表达增加。结论姜黄素能抑制A375细胞增殖和诱导其凋亡,c-myc和Caspase-3基因可能参与凋亡过程。

Abstract: Objective To investigate the effect of curcumin on cell apoptosis and c-myc and caspase-3 expressions in human melanoma A375 cell line. Methods A375 cells were exposed to curcumin treatment and growth inhibition of the cells was examined by MTT assay. Annexin V/propidium iodide double staining and DNA fragmentation analysis were employed for assay of the cell apoptosis and morphological changes of the cells were observed with inverted microscopy and transmission electron microscopy, respectively. In situ hybridization and SABC immunohistochemistry were performed for detection of the expressions of c-Myc and caspase-3 in the A375 cells. Results Curcumin inhibited the growth of A375 cells in both time-and concentration-dependent manners. After treatment with 30 μmol/L curcumin for 48 h, apoptotic morphological changes were observed in the cells and an oligonucleosomal DNA ladder was clearly visualized in DNA fragmentation analysis. The apoptotic rates of the cells treated with curcumin at the concentration above 20 μmol/L were significantly higher than that of the control cells. c-myc expression level was decreased whereas caspase-3 expression increased with the increase in curcumin concentrations. Conclusion Curcumin can inhibit the proliferation and induce apoptosis of A375 cells in vitro, and the genes encoding c-myc and caspase-3 may play a role in the process.

中图分类号:

Q255
R739.5

邱实¹, 谭升顺¹, 张江安¹, 刘安¹, 袁景奕¹, 饶国洲², 王文勇³. 姜黄素诱导人黑色素瘤A375细胞凋亡以及对c-myc、caspase-3表达的影响[J]. 南方医科大学学报, 2005, 25(12): 1517-1521.

QIU Shi¹, TAN Sheng-shun¹, ZHANG Jiang-an¹, LIU An¹, YUAN Jing-yi¹, RAO Guo-zhou², WANG Wen-yong³. Apoptosis induced by curcumin and its effect on c-myc and caspase-3 expressions in human melanoma A375 cell line[J]. Journal of Southern Medical University, 2005, 25(12): 1517-1521.

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