南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (12): 1511-1513,1516.

• • 上一篇    下一篇

双向排斥反应联体动物模型的建立

熊兵1, 陈华德1, 赖文1, 熊仕秋2, 郑少逸1, 高辉1, 卞徽宁1, 刘族安1   

  1. 1. 广东省人民医院烧伤科, 广东, 广州, 510080;
    2. 中山大学基础医学院免疫教研室, 广东, 广州, 510080
  • 出版日期:2005-12-20 发布日期:2005-12-20
  • 基金资助:
    收稿日期:2005-8-13。
    基金项目:广东省医学科学基金(A2002043)
    作者简介:熊兵(1971- ),男,2001年毕业于华中科技大学,硕士,主治医师,电话:020-83827812-61210,E-mail:xiongbingl@hotmail.com.

Establishing a parabiosis animal model of two-way paradigm

XIONG Bing1, CHEN Hua-de1, LAI Wen1, XIONG Shi-qiu2, ZHEN Shao-yi1, GAO Hui1, BIAN Hui-ning1, LIU Zu-an1   

  1. 1. 广东省人民医院烧伤科, 广东, 广州, 510080;
    2. 中山大学基础医学院免疫教研室, 广东, 广州, 510080
  • Online:2005-12-20 Published:2005-12-20

PDF

1278

摘要： 目的 探讨在异基因成年小鼠之间建立联体模型用以研究双向排斥反应。方法 54只雄性C57BL/6(H-2b,C57)小鼠和54只雌性Balb/c(H-2d)小鼠配对分为3组:1组(注射生理盐水)、2组(注射脾细胞+环磷酰胺)、3组(注射脾细胞+环磷酰胺+环孢素A)。于术中经尾静脉注入对方的脾细胞;术前3d和术后1周每日分别腹腔注射环孢素A(30mg·kg·d-1);术后2d腹腔注射环磷酰胺(150mg/kg)1次。对经以上处理的部分联体小鼠在术后1周后分开,相互植皮。观察联体维持时间,并分别检测其混合淋巴细胞反应和诱导迟发型超敏反应(DTH)。结果 第3组联体时间明显延长;第3组联体小鼠DTH,2、3组显示明显抑制,但3组小鼠抑制程度明显比第2组小鼠深。结论 应用免疫抑制剂,可成功建立双向排斥反应联体动物模型。

Abstract: Objective To establish a parabiosis model between allogenic conspecific adult mice to study two-way paradigm. Methods Fifty-four female Balb/c mice and 54 male C57BL/6 mice were paired and equally divided into 3 groups, namely group 1 with normal saline (NS) injection, group 2 with injections of spleen cells and cyclophosphamide (CP), and group 3 injected with spleen cells, CP, and cyclosporin A (CsA). The treatments were performed by injecting the spleen cells from one of the mice in a pair into the other via tail vein and vise versa, and two days after the operation, CP (150 mg/kg) was injected intraperitoneally. Intraperitoneal CsA (30 mg/kg daily) injection was given starting from 2 days before till 7 days after the operation. Twelve of the 18 pairs of parabiosis mice in each group were separated after 1 week, and part of the skin were transplanted to each other. The maintenance of parabiosis was observed in the other 6 pairs of parabiosis mice were observed. Mixed lymphocyte reaction (MLR) and delayed-type hypersensitivity (DTH) were observed and studied in the separated mice. Result The duration of parabiosis maintenance and skin survival of the group 3 was significantly longer than those in the other two groups, and group 3 showed suppressed MLR and DTH. Conclusion With the application of immunosuppressants, we have successfully established the two-way paradigm model in mice.

中图分类号: