南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (12): 1485-1487.

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丝裂霉素C磁性纳米球对肝细胞L-02的细胞增殖和细胞凋亡的影响

任非, 杨西晓, 陈建海   

  1. 南方医科大学南方医院药学部, 广东, 广州, 510515
  • 出版日期:2005-12-20 发布日期:2005-12-20
  • 基金资助:
    收稿日期:2005-8-13。
    基金项目:国家“863”项目(2002AA214121);广东省科技攻关项目(2002C30103)
    作者简介:任非(1974- ),女,2004年毕业于第一军医大学,博士,电话:020-62787236,E-mail:renfeisky@tom.com.

Effect of magnetic mitomycin C nanoparticles on proliferation and apoptosis of L-02 cells in vitro

REN Fei, YANG Xi-xiao, CHEN Jian-hai   

  1. 南方医科大学南方医院药学部, 广东, 广州, 510515
  • Online:2005-12-20 Published:2005-12-20

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摘要： 目的 研究丝裂霉素C-聚氰基丙烯酸正丁酯磁性纳米球(MMC-MPBCA-NP)、聚氰基丙烯酸正丁酯磁性纳米球(MPBCA-NP)和磁流体(MF)以及MMC对正常人肝细胞株(L-02细胞)增殖和凋亡的影响。方法 通过流式细胞术(FCM)检测MMC-MPBCA-NP、MPBCA-NP、MMC、MF对L-02细胞凋亡和细胞周期的影响;通过MTT比色法测定MMC-MPBCA-NP、MPBCA-NP、MF、MMC对L-02细胞增殖的影响。结果 除MF外,MMC-MPBCA-NP、MPBCA-NP、MMC对L-02细胞增殖均有不同程度的抑制作用。不同浓度的MMC-MPBCA-NP、MPBCA-NP、MMC对L-02细胞的增殖均表现浓度依赖性细胞毒性作用,随着浓度的增加与空白组相比MMC、MMC-MPBCA-NP、MPBCA-NP细胞毒性随之增加。结论 上述药物对L-02细胞的增殖的影响:MF>MPBCA-NP>MMC-MPBCA-NP>MMC。MMC-MPBCA-NP和MMC表现增殖抑制作用同时改变L-02细胞的细胞周期,MPBCA-NP表现增殖抑制作用但不改变L-02细胞的细胞周期。

Abstract: Objective To investigate the effects of mitomycin magnetic polybutylcyanoacrylate nanoparticles (MMC-MPBCA-NP), magnetic polybutylcyanoacrylate nanoparticles (MPBCA-NP) and magnetic fluids (MF) and MMC on the proliferation and apoptosis of normal human liver cell strain (L-02 cells) in vitro. Methods The effect of MMC, MMC-MPBCA-NP, MPBCA-NP, and MF on the apoptosis and cell cycle of L-02 cells was studied by flow cytometry. The changes in the cell proliferation in response to treatments of the preparations and agents were examined by MTT colorimetric assay. Results MMC, MMC-MPBCA-NP, MPBCA-NP produced inhibition of the cell proliferation to different degrees but MF failed to result in cell proliferation inhibition. The results of MTT assay showed that MM, MPBCA-NP and MMC-MPBCA-NP all possessed concentration-dependent cytotoxicity, which increased with the concentration increment in comparison with the blank control. Conclusion The descending order of the 4 agents in their effect of L-02 cell strain growth inhibition is MF >MPBCA-NP>MMC-MPBCA-NP>MMC. MMC and MMC-MPBCA-NP can inhibit the proliferation and cause cell cycle changes of L-02 cells, while MPBCA-NP inhibits the cell proliferation without inducing cell cycle alterations.

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