L型钙通道α1C亚单位在成年大鼠心脏中的表达

摘要/Abstract

摘要： 目的研究L型钙通道α1C亚单位在成年大鼠心脏中的空间表达情况。方法制备成年大鼠心脏冰冻切片,结合大体位置和HE染色结果快速辨认出窦房结、房室结和结后延伸。采用免疫荧光和免疫印迹技术检测L型钙通道α1C亚单位在大鼠窦房结、房室结、结后延伸、右房、右室的表达,用共聚焦显微镜观察其表达部位,通过凝胶成像及图像分析系统对实验结果进行分析。结果荧光呈现点状、斑状或不规则的短线状,主要分布于细胞膜及细胞内的膜性结构,其荧光强度由弱到强依次为窦房结、房室结、结后延伸、右心房、右心室,房室结和结后延伸之间无显著性差异(P>0.05),其余两两相比均有显著差异(P<0.05),其中右心房、右心室明显强于窦房结、房室结和结后延伸(P<0.01)。各部位在相对分子质量200000左右可见一特异性蛋白条带。对蛋白条带进行灰度值分析,结果与免疫荧光相一致。结论 L型钙通道α1C亚单位在成年大鼠心脏分布广泛,在不同部位表达量不同,在工作肌组织的表达量明显高于传导组织,提示钙电流在心脏不同部位有不同的分子基础。

Abstract: Objective To investigate the expression and distribution of L-type calcium channel α1C subunits in adult rat heart. Methods HE staining was applied on the frozen sections of adult rat heart to identify the sinoatrial node (SAN), atrioventricular node (AVN), and posterior nodal extension (PNE). The protein expression of L-type calcium channel α1C in adult rat heart and its cellular localization were examined by Western blotting and immunohistochemnistry, respectively. Results L-type calcium channel α1C subunit was immunolocalized on the membrane of the myocardial cells, and its expression increased gradually in the SAN, AVN, PNE, right atrium and right ventricle. The protein level of L-type calcium channel α1C in the AVN was similar to that in the PNE (P>0.05), and its level in the right atrium and ventricle were significantly higher than those in the SAN and AVN (P<0.01). The results of Western blotting were well consistent with the results of immunohistochemistry. Conclusion The expression of L-type calcium channel α1C subunit may play a role in the electrophysiological functions of the heart.

中图分类号:

欧妍¹, 牛小麟¹, 任付先¹, 张颖¹, 凌凤东². L型钙通道α1C亚单位在成年大鼠心脏中的表达[J]. 南方医科大学学报, 2005, 25(11): 1400-1404.

OU Yan¹, NIU Xiao-lin¹, Ren Fu-xian¹, Zhang Ying¹, Ling Feng-dong². Expression of L-type calcium channel α1C subunit in adult rat heart[J]. Journal of Southern Medical University, 2005, 25(11): 1400-1404.

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