活性氧促使L6成肌细胞的分化

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (11): 1384-1386.

活性氧促使L6成肌细胞的分化

邱小忠¹, 余磊¹, 廖华¹, 张黎声², 秦建强¹, 杨俊¹, 欧阳钧¹

1. 南方医科大学临床解剖学研究所, 广东, 广州, 510515;
2. 上海中医药大学解剖教研室, 上海, 200032

出版日期:2005-11-20 发布日期:2005-11-20
基金资助:
收稿日期:2005-4-10。
基金项目:广东省专项课题(A302020401);广东省科技攻关项目(2KM05503S);广州市科技攻关项目(2002Z1-E0037)
作者简介:邱小忠(1968-),男,讲师,博士,主要从事组织工程研究,电话:020-61647752,E-mail:qqiuxzh@yahoo.com.cn.
通讯作者:欧阳钧,男,博士,副教授

Oxidative stress-induced differentiation of L6 myoblasts

QIU Xiao-zhong¹, YU Lei¹, LIAO Hua¹, ZHANG Li-sheng², QIN Jian-qiang¹, YANG Jun¹, OUYANG Jun¹

1. 南方医科大学临床解剖学研究所, 广东, 广州, 510515;
2. 上海中医药大学解剖教研室, 上海, 200032

Online:2005-11-20 Published:2005-11-20

摘要/Abstract

摘要： 目的探讨氧化应激对L6成肌细胞生长分化的影响。方法 MTT法检测H₂O₂对L6细胞活力的影响;观察H₂O₂引起的细胞形态学变化,RT-PCR检测myogenin基因表达水平。结果在较短的处理时间内(1h),低浓度的活性氧(50μmol/LH₂O₂)有利于细胞的生长(P<0.05);H₂O₂处理12h后,50和150μmol/L的H₂O₂能够诱导成肌细胞分化的标记分子-myogenin基因的表达,形态学研究结果表明H₂O₂能够诱导肌管的形成,促使成肌细胞的分化。结论活性氧可能是细胞内诱导细胞生长与分化的信号分子。

Abstract: Objective To explore the relationship between the differentiation of L6 myoblasts and oxidative stress. Methods MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H₂O₂-induced morphological changes of the cells were observed. Results The myoblasts treated with low concentration of reactive oxygen (50 μmol/L H₂O₂) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 μmol/L H₂O₂ induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H₂O₂. Conclusion The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.

中图分类号:

R-33

邱小忠¹, 余磊¹, 廖华¹, 张黎声², 秦建强¹, 杨俊¹, 欧阳钧¹. 活性氧促使L6成肌细胞的分化[J]. 南方医科大学学报, 2005, 25(11): 1384-1386.

QIU Xiao-zhong¹, YU Lei¹, LIAO Hua¹, ZHANG Li-sheng², QIN Jian-qiang¹, YANG Jun¹, OUYANG Jun¹. Oxidative stress-induced differentiation of L6 myoblasts[J]. Journal of Southern Medical University, 2005, 25(11): 1384-1386.

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