南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (11): 1384-1386.

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活性氧促使L6成肌细胞的分化

邱小忠1, 余磊1, 廖华1, 张黎声2, 秦建强1, 杨俊1, 欧阳钧1   

  1. 1. 南方医科大学临床解剖学研究所, 广东, 广州, 510515;
    2. 上海中医药大学解剖教研室, 上海, 200032
  • 出版日期:2005-11-20 发布日期:2005-11-20
  • 基金资助:
    收稿日期:2005-4-10。
    基金项目:广东省专项课题(A302020401);广东省科技攻关项目(2KM05503S);广州市科技攻关项目(2002Z1-E0037)
    作者简介:邱小忠(1968-),男,讲师,博士,主要从事组织工程研究,电话:020-61647752,E-mail:qqiuxzh@yahoo.com.cn.
    通讯作者:欧阳钧,男,博士,副教授

Oxidative stress-induced differentiation of L6 myoblasts

QIU Xiao-zhong1, YU Lei1, LIAO Hua1, ZHANG Li-sheng2, QIN Jian-qiang1, YANG Jun1, OUYANG Jun1   

  1. 1. 南方医科大学临床解剖学研究所, 广东, 广州, 510515;
    2. 上海中医药大学解剖教研室, 上海, 200032
  • Online:2005-11-20 Published:2005-11-20

摘要: 目的 探讨氧化应激对L6成肌细胞生长分化的影响。方法 MTT法检测H2O2对L6细胞活力的影响;观察H2O2引起的细胞形态学变化,RT-PCR检测myogenin基因表达水平。结果 在较短的处理时间内(1h),低浓度的活性氧(50μmol/LH2O2)有利于细胞的生长(P<0.05);H2O2处理12h后,50和150μmol/L的H2O2能够诱导成肌细胞分化的标记分子-myogenin基因的表达,形态学研究结果表明H2O2能够诱导肌管的形成,促使成肌细胞的分化。结论 活性氧可能是细胞内诱导细胞生长与分化的信号分子。

Abstract: Objective To explore the relationship between the differentiation of L6 myoblasts and oxidative stress. Methods MTT assay was used to determine the viability of L6 myoblasts, from which the total RNA was extracted for amplification of the myogenin gene fragment by RT-PCR. H2O2-induced morphological changes of the cells were observed. Results The myoblasts treated with low concentration of reactive oxygen (50 μmol/L H2O2) for 1 h exhibited accelerated cell growth (P<0.05), and treatment with 50 and 150 μmol/L H2O2 induced the gene expression of myogenin, a molecular marker for differentiation of myoblasts. Morphological study revealed myotube formation and accelerated differentiation of the myoblasts induced by H2O2. Conclusion The reactive oxygen may serve as the intracellular signal molecules to induce the growth and differentiation of the myoblasts.

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