南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (11): 1361-1364.

• • 上一篇    下一篇

霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区序列分析

芮勇宇1, 阚飙2, 高守一2, 刘延清2, 祁国明2   

  1. 1. 南方医科大学南方医院检验科, 广东, 广州, 510515;
    2. 中国疾病预防控制中心传染病预防控制所, 北京, 102206
  • 出版日期:2005-11-20 发布日期:2005-11-20
  • 基金资助:
    收稿日期:2005-4-7。
    基金项目:国家自然科学基金(30470099;30170821)
    作者简介:芮勇宇(1970-),男,副研究员,博士后,硕士导师,电话:020-61642145,E-mail:yongyurui@tom.com

Sequence analysis of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain

RUI Yong-yu1, KAN Biao2, GAO Shou-yi2, LIU Yan-qing2, QI Guo-ming2   

  1. 1. 南方医科大学南方医院检验科, 广东, 广州, 510515;
    2. 中国疾病预防控制中心传染病预防控制所, 北京, 102206
  • Online:2005-11-20 Published:2005-11-20

摘要: 目的 对霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区进行序列分析。方法 采用聚合酶链反应、基因测序及序列分析。结果 JS94484株CTXEVCφ基因组的ig1、rstR、ig2和ctxAB基因与EVC标准株N16961的高度同源。JS94484株nct-CTXnewφ基因组的ig1、rstR和ig2基因,与目前发现的3种类型的同源性均较低,趋异性均较高。CTXEVCφ和nct-CTXnewφ基因组zot基因末端约60bp的基因序列差别较大,推测的氨基酸序列差别也较大。JS94484株的tcpA基因序列与EVC标准株N16961的完全一致。结论 霍乱弧菌nct-CTXnewφ基因组的ig1、rstR和ig2基因为新类型,基因功能有待于进一步研究。

Abstract: Objective To analyze the sequences of the mutated genes in CTXEVCφ and nct-CTXnewφ genomes in Vibrio cholerae JS94484 strain. Methods The mutated genes in CTXEVCφ and nct-CTXnewφ genome were obtained by PCR, sequenced and analyzed. Results ig1, rstR, ig2, and ctxAB genes in CTXEVCφ genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTXnewφ genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTXEVCφ and nct-CTXnewφ genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961. Conclusion ig1, rstR and ig2 genes of nct-CTXnewφ genome are of a novel type, and their functions await further investigation.

中图分类号: