霍乱弧菌JS94484株CTXEVCφ和nct-CTXnewφ基因组变异区序列分析

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (11): 1361-1364.

霍乱弧菌JS94484株CTX^EVC_φ和nct-CTX^new_φ基因组变异区序列分析

芮勇宇¹, 阚飙², 高守一², 刘延清², 祁国明²

1. 南方医科大学南方医院检验科, 广东, 广州, 510515;
2. 中国疾病预防控制中心传染病预防控制所, 北京, 102206

出版日期:2005-11-20 发布日期:2005-11-20
基金资助:
收稿日期:2005-4-7。
基金项目:国家自然科学基金(30470099;30170821)
作者简介:芮勇宇(1970-),男,副研究员,博士后,硕士导师,电话:020-61642145,E-mail:yongyurui@tom.com

Sequence analysis of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain

RUI Yong-yu¹, KAN Biao², GAO Shou-yi², LIU Yan-qing², QI Guo-ming²

1. 南方医科大学南方医院检验科, 广东, 广州, 510515;
2. 中国疾病预防控制中心传染病预防控制所, 北京, 102206

Online:2005-11-20 Published:2005-11-20

摘要/Abstract

摘要： 目的对霍乱弧菌JS94484株CTX^EVC_φ和nct-CTX^new_φ基因组变异区进行序列分析。方法采用聚合酶链反应、基因测序及序列分析。结果 JS94484株CTX^EVC_φ基因组的ig1、rstR、ig2和ctxAB基因与EVC标准株N16961的高度同源。JS94484株nct-CTX^new_φ基因组的ig1、rstR和ig2基因,与目前发现的3种类型的同源性均较低,趋异性均较高。CTX^EVC_φ和nct-CTX^new_φ基因组zot基因末端约60bp的基因序列差别较大,推测的氨基酸序列差别也较大。JS94484株的tcpA基因序列与EVC标准株N16961的完全一致。结论霍乱弧菌nct-CTX^new_φ基因组的ig1、rstR和ig2基因为新类型,基因功能有待于进一步研究。

Abstract: Objective To analyze the sequences of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain. Methods The mutated genes in CTX^EVC_φ and nct-CTX^new_φ genome were obtained by PCR, sequenced and analyzed. Results ig1, rstR, ig2, and ctxAB genes in CTX^EVC_φ genome of V. cholerae strain JS94484 were highly homologous with those of standard EVC strain N16961, while ig1, rstR and ig2 genes in nct-CTX^new_φ genome of the strain JS94484 shared low homology with those of the other 3 biotypes of V. cholerae. Considerable difference was detected in the last 60 bp of zot genes between CTX^EVC_φ and nct-CTX^new_φ genomes, indicating possible difference in the amino sequences of the Zot proteins encoded by these two genes. The sequence of toxin-coregulated pilus A subunit gene (tcpA) of the strain JS94484 was identical with that of strain N16961. Conclusion ig1, rstR and ig2 genes of nct-CTX^new_φ genome are of a novel type, and their functions await further investigation.

中图分类号:

R379

芮勇宇¹, 阚飙², 高守一², 刘延清², 祁国明². 霍乱弧菌JS94484株CTX^EVC_φ和nct-CTX^new_φ基因组变异区序列分析[J]. 南方医科大学学报, 2005, 25(11): 1361-1364.

RUI Yong-yu¹, KAN Biao², GAO Shou-yi², LIU Yan-qing², QI Guo-ming². Sequence analysis of the mutated genes in CTX^EVC_φ and nct-CTX^new_φ genomes in Vibrio cholerae JS94484 strain[J]. Journal of Southern Medical University, 2005, 25(11): 1361-1364.

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