白术内酯Ⅰ对肿瘤恶病质患者细胞因子和肿瘤代谢因子的影响

摘要/Abstract

摘要： 目的观察白术内酯Ⅰ对64例经组织学确诊的晚期恶性肿瘤恶病质患者血清细胞因子IL-1、IL-6及TNF-α和肿瘤代谢因子蛋白水解诱导因子(PIF)的影响。方法应用免疫组化和Western-blot技术进行随机病例对照研究,观察白术内酯Ⅰ对肿瘤恶病质患者的食欲、上臂肌肉周径(MAMC)、消瘦及体力状况、细胞因子IL-1、TNF-α以及尿中蛋白水解诱导因子PIF水平的影响。结果白术内酯Ⅰ可以显著改善恶病质患者的食欲、上臂肌肉周径(MAMC)、消瘦及体力状况。同时可以显著降低细胞因子IL-1、TNF-α以及尿中蛋白水解诱导因子PIF的水平。但其对增加体质量以及降低IL-6水平效果不显著。结论白术内酯Ⅰ是有前景的抗肿瘤恶病质药物。

Abstract: Objective To observe the effect of lactoneⅠfrom Atractylodes macrocephala Koidz (LAMK I) on the cytokines and proteolysis-inducing factors (PIF) in cachectic cancer patients. Method Sixty-four cachectic cancer patients were randomized into two groups, namely LAMK I group and FOE (fish oil-enriched nutritional supplementation) treatment group. The appetite, body weight changes, mid-arm muscle circumference (MAMC), and KPS scores were recorded 3 and 7 weeks after the treatment. Immumohistochemistry was used to analyze the changes in the cytokines interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF)-α and Western blotting employed to examine the changes of PIF after the treatment. Results The patients’ appetite and MAMC in LAMKI group was better than those of patients in FOE group, and the serum IL-1 and TNF-α levels and urine PIF level were significantly lower than those of FOE group. Body weight and serum IL-6 level were not significantly different between the two groups. Conclusion LactoneⅠfrom Atractylodes macrocephala Koidz can be beneficial for treating cancer cachexia.

中图分类号:

R285
R730.52

刘昳, 叶峰, 邱根全, 章梅, 王锐, 何群英, 蔡云. 白术内酯Ⅰ对肿瘤恶病质患者细胞因子和肿瘤代谢因子的影响[J]. 南方医科大学学报, 2005, 25(10): 1308-1311.

LIU Yi, YE Feng, QIU Gen-quan, ZHANG Mei, WANG Rui, HE Qun-ying, CAI Yun. Effects of lactoneⅠfrom Atractylodes macrocephala Koidz on cytokines and proteolysis-inducing factors in cachectic cancer patients[J]. Journal of Southern Medical University, 2005, 25(10): 1308-1311.

参考文献

[1] 郑广娟.白术对小鼠S180肉瘤的抑瘤作用及肿瘤凋亡相关基因bcl-2表达的影响[J].生物医学工程研究,2003,3:48-50.Zheng GJ. Baizhu’ s effect of suppressing tumors on S 180 sarcoma and the effect of Baizhu on metastasis - associated gene bcl-2 [J]. J Biomed Engineering Res, 2003,3: 48-50.
[2] 国家药典委员会·中国药典[M].2000一部,北京:化学工业出版社,2000.77.
[3] 汤得芳,郝玉巧,刘佐雅,等.平江白术根茎挥发油的成分及其抑瘤作用研究[J].药学通报,1984,19(9):43.
[4] 张奕强,许实波,林永成.白术内酯系列物的胃肠抑制作用[J].中药材,1999,22(12):636-40.Zhang YQ, Xu SB, Lin YC. Gastrointestinal inhibitory effects of sesquiterpene lactones from Atractylodes macrocephala [J]. Tradit Herb, 1999, 22(12): 636-40.
[5] Jelliffe DB. The assessment of the nutritional status of the community[C]. WHO Monograph 53. WHO: Geneva.
[6] Simons JP, Aaronson NK, Vansteenkiste JF, et al. Effects of medroxy progester one acetate on appetite, weight, and quality of life in advanced-stage non-hormone sensitive cancer: A placebocontrolled multicenter study [J]. J Clin Oncol, 1996, 14:1077-84.
[7] Wigmore SJ, Todorov PT, Barber MD, et al. Characteristics of patients with pancreatic cancer expressing a novel cancer cachectic factor[J]. Br J Surg, 2000, 87: 53-8.
[8] 卜俊国,袁亚维,陈俊.x射线照射后鼻咽癌细胞多药耐药基因的表达[J].第一军医大学学报,2004,24(6):646-9.Bu JG, Yuan YW, Chen J. Expression of multidrug resistance gene in nasopharyngeal carcinoma cells after irradiation exposure [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue bao, 2004, 24 (6):646-9.
[9] 高蕾,南清振,白岚,等.食道癌患者血清可溶性肿瘤坏死因子受体1测定及临床意义[J].第一军医大学学报,2000,20(3):260-1.Gao L, Nan QZ, Bai L, et al. Determination of the levels of serum soluble tumor necrosis factor receptor 1 in esophageal carcinoma patients [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue bao,2000, 20 (3): 260-1.
[10] Mantovani G, Maccio A, Mura L, et al. Serum levels of leptin and proinflammatory cytokines in patients with advanced-stage cancer at different sites[J]. J Mol Med, 2000, 78: 554-61.
[11] Rubin H. Cancer cachexia: its correlations and causes [J]. Proc Natl Acad Sci USA, 2003, 100: 5384-9.
[12] Argiles JM, Busquets S, Lopez-Soriano FJ. Cytokines in the pathogenesis of cancer cachexia [J]. Curr Opin Clin Nutr Metab Care,2003, 6: 401-6.
[13] Warne JP. Tumour necrosis factor [alpha]: a key regulator of adipose tissue mass [J]. J Endocrinol, 2003, 177:351-5.
[14] Tisdale MJ. Protein loss in cancer cachexia [J]. Science, 2000, 289:2293-4.
[15] Pamela M. Fish oils-an update[J]. Pharm J, 2000, 265(7122): 720.