聚氰基丙烯酸正丁酯纳米粒的生物相容性研究

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (10): 1261-1263.

聚氰基丙烯酸正丁酯纳米粒的生物相容性研究

杨西晓, 陈建海, 郭丹

南方医科大学南方医院药学部, 广东, 广州, 510515

出版日期:2005-10-20 发布日期:2005-10-20
基金资助:
收稿日期:2005-5-11。
基金项目:863计划资助项目(2002AA214121);广东省重点基金项目(2002C30103)
作者简介:杨西晓(1968- ),女,博士,主管药师,电话:020-61641888-87236,E-mail:yaxx@263.net

Study of biocompatibility of polybutylcyanoacrylate nanoparticles

YANG Xi-xiao, CHEN Jan-hai, GUO Dan

南方医科大学南方医院药学部, 广东, 广州, 510515

Online:2005-10-20 Published:2005-10-20

摘要/Abstract

摘要： 目的对药用载体聚氰基丙烯酸正丁酯纳米粒的生物相容性进行系统性评价。方法体外采用MTT法进行细胞增殖测定、溶血试验,体内采用皮下和肌肉内埋植载体的炎症反应试验。结果该载体对细胞属无毒级,无溶血性,在动物体内埋植3个月后载体降解,周围组织无明显炎症反应。结论所制备的聚氰基丙烯酸正丁酯纳米粒载体具有良好的生物相容性。

Abstract: Objective To evaluate the biocompatibility of polybutylcyanoacrylate nanoparticles (PBCA-NP) manufactured by emulsion polymerization technique. Methods MTT assay was performed for evaluation of the cytotoxicity of PBCA-NP (e.g. cell proliferation and hemolytic test) experiments of long-term subcutaneous implantation and implantation in the muscular tissues of PBCA-NP was conducted in rabbits to observe the inflammatory reactions due to the implantation. Results No obvious cytotoxicity or hemolytic reactions were observed (with the hemolytic rate at different concentrations of PBCA-NP<5%). Three months after PBCA-NP implantation, obvious lymphocyte infiltration was not observed in the tissues around the implants which underwent gradual degradation. Conclusion PBCA-NP possesses good biocompatibility.

中图分类号:

R979.1

杨西晓, 陈建海, 郭丹. 聚氰基丙烯酸正丁酯纳米粒的生物相容性研究[J]. 南方医科大学学报, 2005, 25(10): 1261-1263.

YANG Xi-xiao, CHEN Jan-hai, GUO Dan. Study of biocompatibility of polybutylcyanoacrylate nanoparticles[J]. Journal of Southern Medical University, 2005, 25(10): 1261-1263.

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