L-型钙通道α1亚单位在成年鼠海马神经元上的差异性表达

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (10): 1225-1227.

L-型钙通道α1亚单位在成年鼠海马神经元上的差异性表达

杨建明¹, 胡德辉², 朱心红¹, 高天明¹

1. 南方医科大学神经生物学教研室, 广东, 广州, 510515;
2. 南方医科大学生理学教研室, 广东, 广州, 510515

出版日期:2005-10-20 发布日期:2005-10-20
基金资助:
收稿日期:2005-3-15。
基金项目:国家自然科学基金(30125013,30330240);广东省自然科学基金(010639);教育部“长江学者”奖励计划
作者简介:杨建明(1977- ),男,神经生物学专业硕士,电话:020-31778227,E-mail:yangjm@fimmu.com
通讯作者:高天明(1960-),教育部"长江学者"特聘教授,博士生导师,电话:020-61648216,E-mail:tgao@fimmu.com

Differential expression of L-type calcium channel α1 subunits on adult rat hippocampal neurons

YANG Jian-ming¹, HU De-hui², ZHU Xin-hong¹, GAO Tian-ming¹

1. 南方医科大学神经生物学教研室, 广东, 广州, 510515;
2. 南方医科大学生理学教研室, 广东, 广州, 510515

Online:2005-10-20 Published:2005-10-20

摘要/Abstract

摘要： 目的观察L-型钙通道不同的α1亚单位在成年鼠海马不同亚区神经元中表达的变化。方法运用免疫组织化学染色方法特异性地标记脑型L-型L-型钙通道不同的α1亚单位CaV1.2α1C和CaV1.3α1D。结果 CaV1.2α1C主要分布在CA1区锥体细胞突起部分及CA3区锥体细胞的胞体区、基树突和远端顶树突;CaV1.3α1D则主要集中分布在海马各亚区的胞体区和近端突起;此外,CaV1.2α1C和CaV1.3α1D在细胞局部的分布特性上亦明显不同,前者主要呈现簇状分布,而后者则呈均匀分布。结论 L-型钙通道CaV1.2α1C和CaV1.3α1D亚单位在成年鼠海马不同亚区神经元中呈现差异性表达。

Abstract: Objective To study the localization of L-type calcium channel α1 subunits CaV1.2α1C and CaV1.3α1D in CA1 and CA3 regions of adult rat hippocampus. Methods Immunohistochemical staining was employed for specific labeling of α1 subunits CaV1.2α1C and CaV1.3α1D. Results CaV1.2α1C subunit was mainly located in the apical and basal dendrites in the CA1 area and the CA3 area, neuronal soma, basal dendrites and distal apical dendrites were all positively stained. In contrast to CaV1.2α1C, CaV1.3α1D displayed no obvious difference in immunostaining between CA1 and CA3, and was distributed mainly in the neuronal soma and proximal dendrites. At cellular level, CaV1.2α1C distribution showed a distinct clustered pattern while a uniform distribution was observed for CaV1.3α1D subunit. Conclusion Different α1 subunits of L-type calcium channel have differential expression in adult hippocampal neurons.

中图分类号:

R338.26

杨建明¹, 胡德辉², 朱心红¹, 高天明¹. L-型钙通道α1亚单位在成年鼠海马神经元上的差异性表达[J]. 南方医科大学学报, 2005, 25(10): 1225-1227.

YANG Jian-ming¹, HU De-hui², ZHU Xin-hong¹, GAO Tian-ming¹. Differential expression of L-type calcium channel α1 subunits on adult rat hippocampal neurons[J]. Journal of Southern Medical University, 2005, 25(10): 1225-1227.

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