局灶性脑缺血后室管膜 /室下区细胞迁移到梗塞区周围并分化为神经元和星形胶质细胞

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (10): 1201-1206.

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局灶性脑缺血后室管膜 /室下区细胞迁移到梗塞区周围并分化为神经元和星形胶质细胞

张蓬勃^1,2, 刘勇¹, 李捷¹, 康前雁¹, 田英芳¹, 陈新林¹, 赵建军¹, 石秦东¹, 宋土生¹, 钱亦华¹

1. 西安交通大学医学院环境与疾病相关基因教育部重点实验室, 神经科学研究中心, 陕西, 西安, 710061;
2. 西安交通大学第二医院麻醉科, 陕西, 西安, 710004

出版日期:2005-10-20 发布日期:2005-10-20
基金资助:
收稿日期:2005-5-16;改回日期:2005-7-6。
基金项目:This work was supported by Grant from National Natural ScienceFoundation of China (30200291, 30170300) and Natural Science Foundation of Shaanxi Province,China(2002C250,2001SM63).
作者简介:Zhang Peng-bo(1968- ):PhD candidate,attending physician,major in neurobiology,Tel:029-82655428,E-mail:zhpbo@163.com

Ependymal/subventricular zone cells migrate to the peri-infarct region and differentiate into neurons and astrocytes after focal cerebral ischemia in adult rats

ZHANG Peng-bo^1,2, LIU Yong¹, LI Jie¹, KANG Qian-yan¹, TIAN Ying-fang¹, CHEN Xin-lin¹, ZHAO Jian-jun¹, SHI Qin-dong¹, SONG Tu-sheng¹, QIAN Yi-hua¹

1. 西安交通大学医学院环境与疾病相关基因教育部重点实验室, 神经科学研究中心, 陕西, 西安, 710061;
2. 西安交通大学第二医院麻醉科, 陕西, 西安, 710004

Online:2005-10-20 Published:2005-10-20

摘要/Abstract

摘要： 目的研究局灶性脑缺血后室管膜/室下区细胞的迁移分化,揭示梗塞区周围新生细胞的来源。方法大脑中动脉阻塞前,将10μl0.2%的荧光染料DiI注射于体质量250~350g的雄性SD大鼠侧脑室以预标记室管膜/室下区细胞。脑缺血后,采用累积式的BrdU标记方法标记新生细胞并通过双重免疫荧光染色确定细胞分化。标记的细胞通过激光共聚焦显微镜观察。结果在非缺血对照大鼠,DiI标记细胞定居于室管膜/室下区。局灶性脑缺血后,DiI标记细胞出现于胼胝体,邻近的纹状体和皮质。此外,缺血14d后,梗塞区周围纹状体和皮质内可见一些DiI/BrdU/GFAP或DiI/BrdU/NeuN三重标记阳性细胞。结论局灶性脑缺血后,室管膜/室下区细胞迁移到梗塞区周围并分化成神经元和星形胶质细胞,这一发现对于理解成体神经干细胞的起源和开发促进脑损伤后内源性神经发生的新措施具有重要意义。

Abstract: Objective To investigate the migration and differentiation of ependymal/ subventricular zone cells after focal cerebral ischemia in rats, and reveal the origin of the newly generated neural cells in the peri-infarct region. Methods Normal adult male Sprague Dawley rats weighing 250-350 g were used in this study. Before middle cerebral artery occlusion (MCAO), 10 μl of 0.2% DiI was injected into the lateral ventricle for prelabeling the ependymal/subventricular zone cells. After ischemia, cumulative BrdU labeling was employed to detect the newly generated cells and double immunofluorescent staining to identify cell differentiation. The labeled cells were observed with laser confocal microscopy. Results In the non-ischemic control rats, DiI-labeled cells resided in the ependyma/subventricular zone. After focal cerebral ischemia, DiI-labeled cells were found in the corpus callosum, adjacent striatum and cortex, and some DiI/BrdU/glial fibrillary acidic protein (GFAP)-positive cells or DiI/BrdU/ neuronal nuclear antigen (NeuN)-positive cells were observed in the peri-infarct region in the striatum or cortex since day 14 after MCAO. Conclusion After focal cerebral ischemia, ependymal/subventricular zone cells migrate into the peri-infarct region where they differentiate into neurons and astrocytes. This finding may be important for understanding the source of adult neural stem cells and for developing new therapeutic intervention strategy through enhancing endogenous neurogenesis after brain injury.

中图分类号:

张蓬勃^1,2, 刘勇¹, 李捷¹, 康前雁¹, 田英芳¹, 陈新林¹, 赵建军¹, 石秦东¹, 宋土生¹, 钱亦华¹. 局灶性脑缺血后室管膜 /室下区细胞迁移到梗塞区周围并分化为神经元和星形胶质细胞[J]. 南方医科大学学报, 2005, 25(10): 1201-1206.

ZHANG Peng-bo^1,2, LIU Yong¹, LI Jie¹, KANG Qian-yan¹, TIAN Ying-fang¹, CHEN Xin-lin¹, ZHAO Jian-jun¹, SHI Qin-dong¹, SONG Tu-sheng¹, QIAN Yi-hua¹. Ependymal/subventricular zone cells migrate to the peri-infarct region and differentiate into neurons and astrocytes after focal cerebral ischemia in adult rats[J]. Journal of Southern Medical University, 2005, 25(10): 1201-1206.

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局灶性脑缺血后室管膜 /室下区细胞迁移到梗塞区周围并分化为神经元和星形胶质细胞

Ependymal/subventricular zone cells migrate to the peri-infarct region and differentiate into neurons and astrocytes after focal cerebral ischemia in adult rats

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