糖尿病大鼠血浆肾上腺髓质素的动态变化及依那普利的干预效应

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (09): 1149-1151.

糖尿病大鼠血浆肾上腺髓质素的动态变化及依那普利的干预效应

徐春生¹, 李志梁¹, 张桦², 严全能¹, 吴宏超¹, 唐朝枢³

1. 南方医科大学珠江医院, 心内科, 北京, 100000;
2. 南方医科大学珠江医院, 内分泌科, 广东, 广州, 510282;
3. 北京大学医学院心血管研究所, 北京, 100000

出版日期:2005-09-20 发布日期:2005-09-20
基金资助:
收稿日期:2005-3-3。
基金项目:九七三国家重大基础发展研究项目(G20000569)
作者简介:徐春生(1964- ),男,博士研究生.

Dynamic changes in plasma adrenomedullin levels and effect of enalapril intervention in diabetic rats

XU Chun-sheng¹, LI Zhi-liang¹, ZHANG Hua², YAN Quan-neng¹, WU Hong-chao¹, TANG Chao-shu³

1. 南方医科大学珠江医院, 心内科, 北京, 100000;
2. 南方医科大学珠江医院, 内分泌科, 广东, 广州, 510282;
3. 北京大学医学院心血管研究所, 北京, 100000

Online:2005-09-20 Published:2005-09-20

摘要/Abstract

摘要： 目的观察糖尿病大鼠血浆肾上腺髓质素(ADM)的动态变化,并探讨依那普利对血浆ADM浓度的影响。方法将52只Wistar大鼠分为:1个月对照组和糖尿病组(各7只);3个月对照组和糖尿病组(各7只);5个月对照组、糖尿病组和依那普利治疗组(各8只)。分别于1、3、5月经心脏采集血浆标本,采用放免法检测血浆ADM浓度。结果糖尿病大鼠血浆ADM浓度(pmol/L)于1、3月时显著高于对照组,分别为17.59±9.78vs7.39±3.29、19.09±3.59 vs 7.91±4.74,5月程(10.41±3.97)显著低于1、3月程;依那普利治疗组血浆ADM浓度(22.45±13.77)显著高于同月程未治疗组(10.41±3.97)及对照组(10.30±2.83)。结论 ADM在糖尿病及其慢性并发症的病理生理中可能发挥代偿保护作用。

Abstract: Objective To observe the dynamic changes of plasma adrenomedullin (ADM) levels and the effect of enalapril intervention in diabetic rats. Methods Fifty-two wistar rats were grouped into 1-and 3-month groups (7 each), both including a control and a streptozotocin-induced diabetic group. The 5-month group was divided into control, diabetic and enalapril-treatment diabetic groups (8 each, the last group receiving oral enalapril treatment at the daily dose of 2 mg/kg from the first to the fifth month after diabetes inducion). Blood samples were collected from the heart of the rats at the end of 1, 3 and 5 months for determination of plasma ADM concentrations radioimmunoassay. Results Plasma ADM levels were significantly higher in diabetic rats than in the corresponding control rats in 1-and 3-month groups. ADM levels in the diabetic rats of 5-month group were significantly decreased in comparison with that of 1-and 3-month groups. In 5-month group, plasma ADM levels of enalapril-treated diabetic rats elevated significantly in comparison with that in the control rats and the diabetic rats without enalapril treatment. Conclusion ADM may play an important role in the pathophysiology of diabetes.

中图分类号:

R586

徐春生¹, 李志梁¹, 张桦², 严全能¹, 吴宏超¹, 唐朝枢³. 糖尿病大鼠血浆肾上腺髓质素的动态变化及依那普利的干预效应[J]. 南方医科大学学报, 2005, 25(09): 1149-1151.

XU Chun-sheng¹, LI Zhi-liang¹, ZHANG Hua², YAN Quan-neng¹, WU Hong-chao¹, TANG Chao-shu³. Dynamic changes in plasma adrenomedullin levels and effect of enalapril intervention in diabetic rats[J]. Journal of Southern Medical University, 2005, 25(09): 1149-1151.

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