预刺激淋巴细胞的伸瘤运动

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (09): 1124-1127.

预刺激淋巴细胞的伸瘤运动

杨介钻¹, 马骊¹, 何小维², 周明乾¹, 胡志明¹, 王小宁²

1. 南方医科大学分子免疫研究所, 广东, 广州, 510515;
2. 华南理工大学生物科学与工程学院, 广东, 广州, 510641

出版日期:2005-09-20 发布日期:2005-09-20
基金资助:
收稿日期:2004-3-16。
基金项目:国家重点基础研究发展规划(973)资助(No.2001CB510008)
作者简介:杨介钻(1973- ),男,博士生,讲师,电话:020-61648322,E-mail:yjzl17@163.com
通讯作者:王小宁,电话:020-87114240.

Emperipolesis of pre-stimulated lymphocytes

YANG Jie-zuan¹, MA Li¹, HE Xiao-wei², ZHOU Ming-qian¹, HU Zhi-ming¹, WANG Xiao-ning²

1. 南方医科大学分子免疫研究所, 广东, 广州, 510515;
2. 华南理工大学生物科学与工程学院, 广东, 广州, 510641

Online:2005-09-20 Published:2005-09-20

摘要/Abstract

摘要： 目的对淋巴细胞的伸瘤现象进行描述,并探讨伸瘤的一些影响因素及伸瘤运动与淋巴细胞功能的关系。方法分离外周血淋巴细胞,用PHA、IL-2、a-CD3、PHA+IL-2预刺激后与口腔上皮癌细胞混合作用。于倒置显微镜下直接观察淋巴细胞的粘(伸)瘤情况;取混合细胞经冷丙酮固定并Gimasa染色后在镜下观察淋巴细胞与瘤细胞作用情况,并于1、2、4、6h,取混合淋巴细胞于扫描电镜下观察伸瘤运动的发生、发展变化。结果与未预刺激组比较,PHA组能提高粘瘤指数,IL-2、a-CD3、PHA+IL-2均能提高淋巴细胞的粘(伸)瘤指数,以PHA+IL-2对粘(伸)瘤率的提高作用最为显著,电镜下发现在粘(伸)瘤运动过程中,外周血淋巴细胞的形态也发生变化。结论淋巴细胞预刺激能促进伸瘤运动的发生,伸瘤运动可能是淋巴细胞的一种杀伤方式。

Abstract: Objective To describe the emperipolesis of lymphocytes and investigate the factors affecting lymphocyte emperipolesis in the KB cells and the relation of the emperipolesis to the biological behaviors of the lymphocytes. Methods After pre-stimulation with phytohemagglutinin (PHA), interleukin (IL)-2, a-CD3, and PHA+IL-2, respectively, the peripheral blood lymphocytes (PBLs) isolated from healthy donors were mixed with KB cells to observe the emperipolesis of the PBLs under inverted microscope every other hour, and calculate the tumor-adhesion/emperipolesis indices of the PBLs.The cell mixture was then fixed with cool acetone and stained by Giemasa to observe emperipolesis between PBLs and KB cells. The occurrence and development of emperipolesis between PBLs and KB cells was also observed under scanning electron microscope (SEM) at 1, 2, 4, and 6 hours after fixation. Results The tumor-adhesion/emperipolesis indices of pre-stimulated PBLs were significantly higher than those of non-stimulated PBLs, and the indices of PHA+IL-2 group were the highest. The PBLs underwent morphological changes in the course of PBL emperipolesis mixed with KB cells as observed under SEM. Conclusion Pre-stimulation can enhance the tumor-adhesion/emperipolesis indices of the PBLs. The emperipolesis might be a means of cytotoxicity of the PBLs.

中图分类号:

R73-53

杨介钻¹, 马骊¹, 何小维², 周明乾¹, 胡志明¹, 王小宁². 预刺激淋巴细胞的伸瘤运动[J]. 南方医科大学学报, 2005, 25(09): 1124-1127.

YANG Jie-zuan¹, MA Li¹, HE Xiao-wei², ZHOU Ming-qian¹, HU Zhi-ming¹, WANG Xiao-ning². Emperipolesis of pre-stimulated lymphocytes[J]. Journal of Southern Medical University, 2005, 25(09): 1124-1127.

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