骨髓间质干细胞移植后大鼠缺血心肌的超微结构观察

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (09): 1090-1094.

骨髓间质干细胞移植后大鼠缺血心肌的超微结构观察

蒋文慧, 马爱群, 韩克, 刘宇, 王亭忠, 郑小璞, 王军

西安交通大学第一医院心血管内科/教育部环境与疾病相关基因重点实验室, 陕西, 西安, 710061

出版日期:2005-09-20 发布日期:2005-09-20
基金资助:
收稿日期:2005-3-12。
基金项目:西安交通大学重点培植项目
作者简介:蒋文慧(1970- ),女,讲师,西安交通大学在读博士研究生,研究方向:干细胞与心肌再生,电话:029-85323524,E-mail:jwh-fq@163.com.
通讯作者:马爱群,E-mail:maaiqun108@hotm ail.com.

Ultrastructural changes of rat ischemic myocardium after bone marrow mesenchymal stem cell transplantation

JIANG Wen-hui, MA Ai-qun, HAN Ke, LIU Yu, WANG Ting-zhong, ZHENG Xiao-pu, WANG Jun

西安交通大学第一医院心血管内科/教育部环境与疾病相关基因重点实验室, 陕西, 西安, 710061

Online:2005-09-20 Published:2005-09-20

摘要/Abstract

摘要： 目的观察骨髓间质干细胞(MSCs)移植后大鼠缺血心肌组织的超微结构改变。方法体外培养、纯化SD大鼠的骨髓MSCs,4’6-二脒-2-苯基吲哚(DAPI)标记,经尾静脉注射移植治疗大鼠急性心肌缺血,移植后1周和8周荧光显微镜下检测心肌组织中DAPI阳性细胞,透射电镜观察心肌组织的超微结构改变。结果 MSCs经传代培养至第3代后,均一性好。细胞移植后1周和8周,移植组的心肌组织中可见呈蓝色细胞核的DAPI阳性细胞,而对照组未发现阳性细胞。移植后1周,心肌组织的超微结构观察发现移植组的心肌梗死周边区域可见少量细胞,其形态类似于体外培养的骨髓MSCs;移植后8周,移植组梗死周边可见大量的毛细血管,其内皮细胞细胞“芽生”分割管腔开始形成新的血管,还可见少量“不成熟”的心肌细胞。结论骨髓MSCs通过血液循环可向缺血心肌组织归巢并促进血管和心肌组织的再生。

Abstract: Objective To observe the ultrastructural changes of ischemic myocardium of rats after bone marrow mesenchymal stem cells (MSCs) transplantation. Methods Rat models of myocardial ischemia were established by ligating the descending anterior branch of the left coronary artery. The isolated and in vitro cultured MSCs labeled with 4’ 6-diamidine-2-phenylindole (DAPI) were injected into the rats via the tail vein and the hearts of the rats were taken 1 week and 8 weeks after transplantation, respectively, for observation under fluorescence microscopy. The ultrastructural changes of the ischemic myocardium of the recipient rats were observed by transmission electron microscope. Results The third passage of cultured MSCs growing in colonies possessed good homogeneity. One week and 8 weeks after transplantation, DAPI-labeled cells were observed in the heart of the recipient rats, but not in the hearts of control rats. One week after transplantation, ultrastructural observation identified a small number of cells in the peripherals of the infarct area of the recipient rats with similar morphology to that of MSCs cultured in vitro. At 8 weeks,a large number of capillaries were seen in the ischemic myocardium on the peripheral of the infarct area. Ultrastructural observation also revealed some immature myocytes surviving in the ischemic region. Conclusion MSCs is capable in vivo of homing to the ischemic myocardium via the blood circulation, and promote regeneration of the myocardium and blood vessels.

中图分类号:

R246

蒋文慧, 马爱群, 韩克, 刘宇, 王亭忠, 郑小璞, 王军. 骨髓间质干细胞移植后大鼠缺血心肌的超微结构观察[J]. 南方医科大学学报, 2005, 25(09): 1090-1094.

JIANG Wen-hui, MA Ai-qun, HAN Ke, LIU Yu, WANG Ting-zhong, ZHENG Xiao-pu, WANG Jun. Ultrastructural changes of rat ischemic myocardium after bone marrow mesenchymal stem cell transplantation[J]. Journal of Southern Medical University, 2005, 25(09): 1090-1094.

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