南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 986-990.

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地塞米松对哮喘豚鼠气管平滑肌毒蕈碱受体mRNA的表达及肺泡灌洗液嗜酸性粒细胞浸润的影响

史亮1, 罗雅玲1, 赖文岩2, 罗亮3   

  1. 1. 南方医科大学南方医院呼吸内科, 广东, 广州, 510515;
    2. 南方医科大学南方医院心内科实验室, 广东, 广州, 510515;
    3. 广州军区总医院急诊科, 广东, 广州510010
  • 出版日期:2005-08-20 发布日期:2005-08-20
  • 基金资助:
    收稿日期:2005-2-13。
    作者简介:史亮(1975-),男,1999年毕业于第一军医大学,现为南方医科大学呼吸内科硕士研究生,医师,电话:020-61641575,E-mai1:shiliangl88@163.com
    通讯作者:罗雅玲,教授,博士研究生导师,研究方向:支气管哮喘的发病机理与临床,电话:020-61641575

Effects of dexamethasone on the expression of muscarinic receptor mRNA in asthmatic guinea pig airway smooth muscle and eosinophil infiltration in bronchoalveolar lavage fluid

SHI Liang1, LUO Ya-ling1, LAI Wen-yan2, LUO Liang3   

  1. 1. 南方医科大学南方医院呼吸内科, 广东, 广州, 510515;
    2. 南方医科大学南方医院心内科实验室, 广东, 广州, 510515;
    3. 广州军区总医院急诊科, 广东, 广州510010
  • Online:2005-08-20 Published:2005-08-20

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摘要： 目的 探讨地塞米松对哮喘豚鼠气管平滑肌毒蕈碱受体(MR)mRNA的表达及肺泡灌洗液(BALF)嗜酸性粒细胞(Eos)浸润的影响。方法 30只健康豚鼠随机分为3组:正常对照组、哮喘组和地塞米松治疗组。后两组制作成哮喘豚鼠模型,再激发用药。测定BALF的细胞总数及细胞分类,观察肺组织的病理改变,进行气管平滑肌的M2R、M3RmRNART-PCR半定量分析。结果 各组BALF的Eos计数,地塞米松治疗组(11.0±3.1)较正常组(2.8±3.0)和哮喘组(29.2±7.3)有显著差异(P<0.01)。地塞米松治疗组肺组织充血水肿明显,但Eos肺组织浸润较哮喘组有明显减少。气管平滑肌的MRmRNART-PCR半定量分析显示,地塞米松治疗组M2R(0.90±0.14)与正常组(0.50±0.16)及哮喘组(1.26±0.24)比较均有显著差异(P<0.01),地塞米松组M3R与哮喘组比较有显著差异(P<0.01),与正常组比较无显著差异;正常组与哮喘组比较,M2R和M3R均有显著差异(P<0.01)。结论 哮喘豚鼠M2R表达增加,M3R表达减少;地塞米松治疗可以通过抑制Eos浸润等炎症反应,调节M2R及M3R表达,从而恢复M2R功能,达到治疗哮喘的目的。

Abstract: Objective To investigate the effect of dexamethasone on the expression of muscarinic receptor (MR) mRNA in smooth muscle and infiltration of eosinophils (Eos) in the airway of asthmatic guinea pigs. Methods Thirty healthy guinea pigs were randomized into 3 equal groups, the control group, asthmatic group and dexamethasone therapy group. Asthma was induced in the latter 2 groups with the asthma-inducing agents and received treatments as indicated. Bronchial alveolar lavage fluid(BALF) were collected subsequently from the guinea pigs for examining the total cell number and cell classification, and histopathologic examination of the lung tissue was performed. Semi-quantitative analysis with reverse transcriptional- polymerase chain reaction (RT-PCR) was performed for M2 and M3 receptor mRNA in airway smooth muscle. Results Compared with the control and the asthmatic group, the number of Eos in the BALF of dexamethasone therapy group was significantly lower (P<0.01). In spite of the presence of hyperemia and edema in the lung tissues of the dexamethasone therapy group, Eos infiltration was less severe than that in the asthmatic group. As found by RT-PCR, the quantity of M2 receptor mRNA in the airway smooth muscle of the dexamethasone therapy group was significantly higher than those in both the control and asthmatic groups (P<0.01), and the quantity of M3 receptor mRNA in the airway smooth muscle of dexamethasone therapy group was significantly higher than that in the asthmatic group, but did not significantly differ from that in the control group. The quantities of M2 and M3 receptor mRNAs in the control group were both significantly higher than that in asthmatic group (P<0.01). Conclusion The expression of M2 receptor is increased in antigen- challenged guinea pigs, and that of M3 receptor decreased. Dexamethasone can treat asthma by inhibiting inflammatory action involving Eos infiltration, regulating the expressions of M2 and M3 receptors and restoring the function of M2 receptor.

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