南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 975-978.

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Photofrin-Herceptin偶联物对肿瘤细胞株的体外杀伤效应

钱新宇, 罗荣城, 李黎波, 廖旺军, 罗宇玲   

  1. 南方医科大学南方医院肿瘤中心, 广东, 广州, 510515
  • 出版日期:2005-08-20 发布日期:2005-08-20
  • 基金资助:
    收稿日期:2005-3-9。
    作者简介:钱新宇(1975-),男,主治医师,在读硕士研究生,主要研究方向为肿瘤生物治疗
    通讯作者:罗荣城,男,南方医科大学南方医院肿瘤中心主任,教授,博士生导师,中国临床肿瘤协作中心(CSCO)常委,电话:020-61641251

Killing effect of Photofrin-Herceptin immunoconjugate on tumor cell lines in vitro

QIAN Xin-yu, LUO Rong-cheng, LI Li-bo, LIAO Wang-jun, LUO Yu-ling   

  1. 南方医科大学南方医院肿瘤中心, 广东, 广州, 510515
  • Online:2005-08-20 Published:2005-08-20

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摘要： 目的 研究Photofrin-Herceptin偶联物对HER-2表达阳性肿瘤细胞的特异性杀伤作用,为进一步的光免疫治疗奠定基础。方法 用碳二亚胺(EDCI)将光敏剂Photofrin与单抗Herceptin偶联,并分别对SK-BR-3、MCF-7、A549这3种HER-2表达程度不同的肿瘤细胞进行体外杀伤实验,用MTT法检测。结果 偶联物的免疫活性较Herceptin降低,但其对HER-2表达阳性的肿瘤细胞的杀伤作用较Photofrin、Herceptin及Herceptin与Photofin混合物明显增强(P<0.05),对于HER-2表达阴性的肿瘤细胞杀伤效果无明显差异。结论 Photofrin-Herceptin偶联物可特异性杀伤HER-2表达阳性的肿瘤细胞。

Abstract: Objective To study the specific killing effect of the Photofrin-Herceptin immunoconjugate on the tumor cells expressing HER-2. Methods Photofrin (porfimer sodium) was covalently coupled to Herceptin (trastuzumab) via [1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimide hydrochloride](EDCI), and the killing effect of this conjugate was evaluated by means of MTT assay on SK-BR-3, MCF-7 and A549 cell lines expressing HER-2 at different levels. Results The conjugate had weaker immunoactivity than Herceptin, but possessed stronger killing effect on the cell lines expressing HER-2 than Photofrin, Herceptin, as well as the mixture of Photofrin and Herceptin (P<0.05). In the cell lines negative for HER-2 expression, the killing effect of the conjugate was similar to that of Photofrin, Herceptin, and their mixture. Conclusion Photofrin-Herceptin immunoconjugate can specifically kill HER-positive cells in vitro.

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