南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 955-958,966.

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大鼠海马区微量注射胃动素对十二指肠移动性复合肌电活动的影响

刘梅1, 董蕾1, 朱文艺2, 崔杨3, 雷莉4   

  1. 1. 西安交通大学第二医院消化科, 陕西, 西安, 710004;
    2. 北京市红十字会急诊抢救中心骨科, 北京, 100085;
    3. 首都医科大学佑安医院人工肝中心, 北京, 100054;
    4. 北京军区总医院干二科, 北京, 100021
  • 出版日期:2005-08-20 发布日期:2005-08-20
  • 基金资助:
    收稿日期:2005-4-6。
    基金项目:国家自然科学基金(30170414)
    作者简介:刘梅(1973-),女,西安交通大学在读博士,E-mail:maybelake@126.com

Effects of intrahippocampal microinjection of motilin on interdigestive migrating myoelectrical complexes of rat duodenum

LIU Mei1, DONG Lei1, ZHU Wen-yi2, CUI Yang3, LEI Li4   

  1. 1. 西安交通大学第二医院消化科, 陕西, 西安, 710004;
    2. 北京市红十字会急诊抢救中心骨科, 北京, 100085;
    3. 首都医科大学佑安医院人工肝中心, 北京, 100054;
    4. 北京军区总医院干二科, 北京, 100021
  • Online:2005-08-20 Published:2005-08-20

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摘要： 目的 探讨海马微量注射胃动素对十二肠消化间期移行性复合肌电活动(migrating myoelectrical complex,MMC)的影响及作用机制。方法 采用中枢立体定位海马微量注射,多道生理记录仪监测十二指肠MMC的方法,观察海马微量注入胃动素(0.37nmol)对十二指肠MMC的影响。结果 海马微量注入胃动素可以使十二指肠MMC时相明显变短,峰电振幅增加,峰电频率增快,但对峰电时相无明显影响;膈下迷走神经切断术可完全阻断海马胃动素对十二指肠MMC的影响;阿托品、酚妥拉明、普萘洛尔不能阻断海马胃动素对十二指肠MMC的影响;抗胃动素血清只能部分阻断海马胃动素对十二指肠MMC的影响。结论 海马外源性胃动素可以使十二指肠MMC的时相、峰电振幅和峰电频率发生变化,这种作用可能是通过海马-下丘脑-脑干-迷走神经通路,由非肾上腺素能非胆碱能神经元作用于十二指肠平滑肌或者促进胃动素的释放而实现的,海马在十二指肠MMC的调节中发挥着重要作用。

Abstract: Objective To explore the effects of intrahippocampal injection of motilin on interdigestive migrating myoelectrical complex (MMC) of rat duodenum. Methods Adult SD rats of either sex were subjected to injection of 0.5 μl motilin (0.74 nmol/μl) through the guiding cannula stereotaxically implanted into the hippocampus previously. Interdigestive MMC of the duodenum was recorded by RM6240B multilead physiological recording system. Results After motilin injection into the hippocampus, duodenal MMC cycle was significantly shortened, but the amplitude and frequency of phase Ⅲ were increased without affecting the duration of phase Ⅲ. The effect of motilin injection into the hippocampus on MMC was completely abolished by subdiaphragmal vagotomy but not affected by intravenously injected atropine, phentolamine or propranolol. Anti-motilin serum could partly abolish the effects of motilin on MMC. Conclusions Motilin injection into the hippocampus have effects on the duration of duodenal MMC cycle and the amplitude and frequency of phase Ⅲ, which may rely on the effect of noncholinergic and nonadrenergic neurons on duodenal smooth muscle or the increase of local motilin via hippocampus-hypothalamus-brain stem-vagus pathway. The hippocampus plays an important role in duodenal MMC.

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