3号染色体短臂21-22区域63个新基因在鼻咽癌组织中的差异表达

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 942-946,950.

3号染色体短臂21-22区域63个新基因在鼻咽癌组织中的差异表达

彭宏¹, 赵彤², 姚开泰²

1. 南方医科大学南方医院耳鼻咽喉科, 广东, 广州, 510515;
2. 南方医科大学肿瘤研究所, 广东, 广州, 510515

出版日期:2005-08-20 发布日期:2005-08-20
基金资助:
收稿日期:2005-4-13。
基金项目:国家自然科学基金(30270739);广东省自然科学基金(010604);全军医药卫生科研基金(300000M201);广东省医学科研基金(A2003444)
作者简介:彭宏,女,主治医师,E-mail:penghong@fimmu.com

Differential expression of 63 novel genes located on chromosome 3p21-22 in nasopharyngeal carcinoma

PENG Hong¹, ZHAO Tong², YAO Kai-tai²

1. 南方医科大学南方医院耳鼻咽喉科, 广东, 广州, 510515;
2. 南方医科大学肿瘤研究所, 广东, 广州, 510515

Online:2005-08-20 Published:2005-08-20

摘要/Abstract

摘要： 目的寻找与鼻咽癌发病密切相关的未知基因。方法对63个定位于3号染色体短臂21-22D3S1609-D3S1295区域的单拷贝并代表未知基因的EST簇进行网上克隆,在获取基因大片段或全长cDNA序列的基础上,应用半定量逆转录-聚合酶链反应检测63个未知基因在4种鼻咽癌细胞株、32例鼻咽部低分化鳞状细胞癌组织和16例鼻咽部慢性炎症组织中的表达状况。结果 49个未知基因在鼻咽癌组织和鼻咽部慢性炎症组织间的表达水平无显著性差异;8个未知基因在鼻咽癌和鼻咽部慢性炎症组织中均不表达;2个未知基因在鼻咽癌组织中表达明显增强;4个未知基因在鼻咽癌组织中表达明显减弱,其中Hs.27566基因在鼻咽癌细胞和组织中均显著低表达或不表达。结论在染色体3p21-22D3S1609-D3S1295区域构建了63个未知基因在鼻咽癌的表达差异谱;筛选出6个在鼻咽癌组织中差异表达的未知基因。

Abstract: Objective To identify novel genes that may participate in the pathogenesis of nasopharyngeal carcinoma (NPC). Methods Sixty-three single copies of expressed sequence tag (EST) clusters mapped in the region of D3S1609-D3S1295 on chromosome 3p21-22 were obtained and assembled into long or full-length cDNA consensus sequence by in silico cloning strategy. Accordingly, these unigene consensus were studied by semi-quantitative reverse transcriptional (RT)-PCR with poorly differentiated squamous cell carcinoma tissues from 32 patients with primary nasopharyngeal carcinoma (NPC) and inflammatory tissues from 1b noncancerous individuals with chronic nasopharyngitis, as well as 4 NPC cell lines (CNE1?CNE2?HNE1 and HNE2). Results Forty-nine unigene ESTs were expressed equally in both NPC and NP, 8 were expressed neither in NPC nor in NP, 2 had higher expression in NPC than in NP, and 4 had lower expression (or undetectable) in NPC than in NP. The expression of Hs.27566 gene was detected in none of the NPC cell lines, and was significantly reduced or undetectable in NPC biopsies in comparison with NP (P<0.001). Conclusion The differential expression of 63 novel genes located on chromosome 3p21-22 in NPC is mapped and 6 novel genes with differential expression in NPC tissues are identified.

中图分类号:

Q343
Q78

彭宏¹, 赵彤², 姚开泰². 3号染色体短臂21-22区域63个新基因在鼻咽癌组织中的差异表达[J]. 南方医科大学学报, 2005, 25(08): 942-946,950.

PENG Hong¹, ZHAO Tong², YAO Kai-tai². Differential expression of 63 novel genes located on chromosome 3p21-22 in nasopharyngeal carcinoma[J]. Journal of Southern Medical University, 2005, 25(08): 942-946,950.

参考文献

[1] Lung ML, Choi CV, Kong H, et al. Microsatellite allelotyping of chinese nasopharyngeal carcinomas. Anticancer Res, 2001, 21 (4B):3081-4.
[2] Lo KW, Teo PM, Hui AB, et al. High resolution allelotype of microdissected primary nasopharyngealcarcinoma[J]. Cancer Res, 2000,60(13): 3348-53.
[3] 尹志华,黄仲曦,刘腾飞,等鼻咽癌和结直肠癌多基因多阶段多途径模型的探讨[J].中华肿瘤杂志(Chin J Tumor),2004,26(3):135-8
[4] Xiong W, Zeng ZY, Xia JH, et al. A susceptibility locus at chromosome 3p21 linked to familial nasopharyngeal carcinoma[J].Cancer Res, 2004, 64(6): 1972-4.
[5] Cheng Y, Poulos NE, Lung ML, et al. Functional evidence for a nasopharyngeal carcinoma tumor suppressor gene that maps at chromosome 3p21.3[J]. Proc Natl Acad Sci USA, 1998, 95(6):3042-7.
[6] Cuthbert AP, Bond J, Trott DA, et al. Telomerase repressor sequences on chromosome 3 and induction of permanent growth arrest in human breast cancer cells[J]. J Natl Cancer Inst, 1999, 911 0:37-45.
[7] Yang Q, Yoshimura G, Mori I, et al. Deletion of chromosome3p14.2-p25 involving the VHL and FHIT genes in conventional renal cell carcinoma[J]. Cancer Res, 2003, 63(2): 455-7.
[8] Powell CA, Bueno R, Borczuk AC, et al. Patterns of allelic loss differ in lung adenocarcinomas of smokers and nonsmokers[J].Lung Cancer, 2003, 39(1): 23-9.
[9] Beder LB, Gunduz M, Ouchida M, et al. Genome-wide analyses on loss of heterozygosity in head and neck squamous cell carcinomas[J]. Lab Invest, 2003, 83(1): 99-105.
[10] Acevedo CM, Henriquez M, Emmert-Buck MR, et al. Loss of heterozygosity on chromosome arms 3p and 6q in microdissected adenocarcinomas of the uterine cervix and adenocarcinoma in situ[J]. Cancer, 2002, 94(3): 793-802.
[11] Yang Q, Yoshimura G, Mori I, et al. Chromosome 3p and breast cancer[J]. J Hum Genet, 2002, 47(9): 453-9
[12] Wistuba Ⅱ, Maitra A, Carrasco R, et al. High resolution chromosome3p, 8p, 9q and 22q allelotyping analysis in the pathogenesis of gallbladder carcinoma[J]. Br J Cancer, 2002, 87(4): 432-40.
[13] Piddini E, Schmid JA, de Martin R, et al. The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9[J]. EMBO J, 2001, 20(15): 4076-87.
[14] Strausberg RL, Feingold EA, Grouse LH, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA, 2002, 99(26): 16899-903.