基因芯片技术分析系统性红斑狼疮患者外周血白细胞基因表达谱的初步研究

摘要/Abstract

摘要： 目的比较系统性红斑狼疮(SLE)患者与正常对照外周血白细胞的基因表达谱的改变,筛选SLE差异表达基因。方法采集静脉血5ml提取总RNA后,反转录合成、标记cDNA探针,与基因芯片杂交后检测。结果 9例患者与正常人对照均有相似差异表达的基因共89个,涉及细胞因子及受体相关基因、免疫相关基因、细胞信号和传递蛋白、蛋白翻译合成、离子通道和运输蛋白、凋亡相关基因、DNA和RNA结合、转录蛋白、细胞的外基质成分等。聚类分析结果显示在不同病人间尽管存在个体差异性,但是在其基因表达变化模式上仍然具有极大的相似性。结论基因芯片技术筛选出的差异表达基因可能为进一步研究SLE的发生和发展以及寻找分子诊断标志和药物治疗靶标提供线索。

Abstract: Objective To identify the differentially expressed genes in systemic lupus erythematosus (SLE) by comparing the gene expression profiles of peripheral leukocytes between SLE patients and healthy controls. Methods The total RNA was extracted from 5 ml peripheral blood of normal subjects and SLE patients, and reversely transcribed in cDNA templates to synthesize cDNA probes labeled for hybridization with the microarray. Results Totally 89 over- or under-expressed genes were identified in 9 SLE patients as compared with the controls. These genes included genes associated with cytokines and their receptors, immunity, cell signal transduction, protein transcription and synthesis, ion channel and transporters, cell apoptosis, DNA and RNA processing, and extracellular matrix etc. Clustering analysis showed that in spite of the individual diversity of the SLE patients, their gene expression profiles were strikingly similar. Conclusion The differentially expressed genes screened with oligonucleotide DNA microarray technique may provide clues for exploring the pathogenesis and progression of SLE, and for identification of potential molecular markers for diagnosis and development of therapeutic drugs.

中图分类号:

Q78
R593.1

吴元胜, 范瑞强, 陈达灿, 禤国维. 基因芯片技术分析系统性红斑狼疮患者外周血白细胞基因表达谱的初步研究[J]. 南方医科大学学报, 2005, 25(08): 929-934.

WU Yuan-sheng, FAN Rui-qiang, CHEN Da-can, XUAN Guo-wei. Gene expression profiling of peripheral leukocytes from patients with systemic lupus erythema- tosus using oligonucleotide DNA microarray[J]. Journal of Southern Medical University, 2005, 25(08): 929-934.

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