南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 1070-1071,1074.

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妊娠高血压患者胎盘组织基质金属蛋白酶-9与肿瘤坏死因子-α的表达

詹瑛, 陈维萍, 叶元华, 楼艳辉   

  1. 青岛大学医学院附属医院, 山东, 青岛, 266003
  • 出版日期:2005-08-20 发布日期:2005-08-20
  • 基金资助:
    收稿日期:2005-5-8。
    作者简介:詹瑛(1965-),女,1996年毕业于第二军医大学,硕士学位,副主任医师,电话:0532-82911321,E-mail:nnns6@yahoo.com.cn

Expression of matrix metalloproteinase-9 and tumor necrosis factor-α in the placenta of patients with pregnancy-induced hypertension

ZHAN Ying, CHEN Wei-ping, YE Yuan-hua, LOU Yan-hui   

  1. 青岛大学医学院附属医院, 山东, 青岛, 266003
  • Online:2005-08-20 Published:2005-08-20

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摘要： 目的 探讨基质金属蛋白酶-9(MMP-9)、肿瘤坏死因子-α(TNF-α)在妊娠期高血压疾病(简称妊高征)发病机制中的作用。方法 采用免疫组化法检测57例妊高征患者和24例正常妊娠产妇胎盘绒毛上皮组织中MMP-9、TNF-α的表达。结果 轻度妊高征组MMP-9的阳性表达与对照组相比,差异无显著性(P>0.05),而中、重度妊高征组阳性表达明显弱于正常妊娠组(P<0.01),且中、重度妊高征组间比较差异也有显著性(P<0.05)。对照组与轻、中、重度妊高征患者胎盘组织TNF-α表达水平比较,差异有显著性,(分别为P<0.05、P<0.01、P<0.01),且妊高征组中不同程度组间比较,差异也有极显著性(P<0.01)。对照组和轻度妊高征组,MMP-9与TNF-α表达无相关性(r=0.287、P>0.05及r=0.382、P>0.05),但中、重度妊高征患者,MMP-9与TNF-α表达水平存在显著的负相关性(r=-0.563、P<0.05及r=-0.681、P<0.01)。结论 妊高征患者MMP-9表达下降,胎盘不完全侵入,胎盘局部缺血、缺氧,TNF-α表达增多,可能是引发妊高征的原因之一。

Abstract: Objective To investigate the role of matrix metalloproteinase-9 (MMP-9) and tumor necrosis factor-α (TNF-α) in the pathogenesis of pregnancy-induced hypertension (PIH). Methods The placenta samples were collected from 57 patients with PIH and 24 normal pregnant women. Immunohisochemical staining was used to examine positive expression of MMP-9 and TNF-α in these samples. Results The positive expression of MMP-9 in the normal group was significantly higher than that in patients with moderate or severe PIH (P<0.01), while no significant difference was found between normal pregnant women and patients with mild PIH (P>0.05), and expression decreased drastically with the progression of PIH (P<0.05). The expression of TNF-α in the patients with mild, moderate and severe PIH groups were significantly higher than that in the normal group (P<0.05, P<0.01 and P<0.01, respectively), and the differences were also significant between the PIH groups of different severities (P<0.01). No correlation was found between MMP-9 and TNF-α expressions in the normal and mild PIH groups (r=0.287, P>0.05; r=0.382, P>0.05), in the patients with moderate and severe PIH, MMP-9 expression showed inverse correlation with TNF-α expression (r=-0.563, P<0.05; r=-0.681, P<0.01). Conclusion Loweed MMP-9 expression in syncytio- trophoblast might result in defective nidation in the placenta where local ischemia and hypoxia cause abnormal TNF-α elevation, which might be one of the important factors inducing PIH.

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