术前淋巴化疗对乳腺癌腋窝转移癌细胞Bcl-2和Bax蛋白表达的影响

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (08): 1001-1004.

术前淋巴化疗对乳腺癌腋窝转移癌细胞Bcl-2和Bax蛋白表达的影响

吴武军¹, 潘承恩¹, 曾健²

1. 西安交通大学第一医院普通外科, 陕西, 西安, 710061;
2. 广西医科大学第一附属医院胃肠腺体外科, 广西, 南宁, 530021

出版日期:2005-08-20 发布日期:2005-08-20
基金资助:
收稿日期:2005-3-21。
作者简介:吴武军(1976-),男,硕士,医师,2004年毕业于广西医科大学,现为西安交通大学第一医院普通外科博士研究生,主要从事胰腺癌的基础和临床研究工作,电话:029-85274650,E-mail:wwj1976495@163.com
通讯作者:曾健,教授,主任医师,硕士生导师,主要从事乳腺癌、甲状腺癌以及胃癌的基础临床工作,电话:0771-5356701

Effect of preoperative lymphatic chemotherapy on Bcl-2 and Bax expression in axillary metastasis of breast cancer

WU Wu-jun¹, PAN Cheng-en¹, ZENG jian²

1. 西安交通大学第一医院普通外科, 陕西, 西安, 710061;
2. 广西医科大学第一附属医院胃肠腺体外科, 广西, 南宁, 530021

Online:2005-08-20 Published:2005-08-20

摘要/Abstract

摘要： 目的观察术前淋巴化疗对乳腺癌腋窝转移淋巴结癌细胞Bcl-2和Bax蛋白表达的影响,结合凋亡变化,探讨淋巴化疗的作用机制。方法将60例Ⅱ-Ⅲ期乳腺癌病人随机分为淋巴化疗组(40例)和对照组(20例)。淋巴化疗组改良根治术前72h于瘤床或肿瘤周围注射表阿霉素-活性炭混悬液10mg,对照组注射表阿霉素水溶液10mg。用末端转移酶标记法检测腋窝转移淋巴结癌细胞凋亡指数AI,用免疫组化SP法检测Bcl-2和Bax蛋白的表达。结果淋巴化疗组转移癌细胞AI平均为(9.5±2.7)%,对照组平均(3.8±1.4)%,前者明显高于后者(P<0.01);淋巴化疗组Bcl-2蛋白表达比对照组升高(P<0.05),而Bax蛋白表达升高更明显(P<0.01),Bcl-2/Bax的比值下降。结论淋巴化疗能诱导乳腺癌腋窝转移淋巴结内癌细胞凋亡增加,其作用可能是通过降低Bcl-2/Bax蛋白的比值实现的。

Abstract: Objective To observe the expression of Bcl-2 and Bax proteins and cell apoptosis induced by preoperative lymphatic chemotherapy with epirubicin-activated carbon suspension (Epi-CH) in the cells of axillary metastatic lymph node of breast cancer and investigate the mechanism. Methods Sixty patients with breast cancer of stages Ⅱ-Ⅲ were randomly divided into two groups. Forty patients in Epi-CH group were injected with 10 mg Epi-CH in the tissue around the primary tumor or biopsy excision 72 h before operation. Twenty patients in the control group were injected with 10 mg epirubicin solution in the same region. The stained lymph nodes full of tumor cells in Epi-CH group and the non-stained nodes in the control group were selected for apoptotic detection by TUNEL method. The expression of Bcl-2 and Bax proteins were examined by SP immunohistochemistry. Results The apoptotic index of the metastatic cancer cells in Epi-CH group was increased remarkably in comparison with that in the control group [(9.5±2.7) % vs (3.8±1.4) %, P<0.01). Compared with the control group, the expression of Bcl-2 and Bax proteins were up-regulated significantly in Epi-CH group (P<0.05 and P<0.01, respectively), resulting in decreased ratio of Bcl-2/Bax. Conclusion Lymphatic chemotherapy can promote cell apotosis in axillary metastasis of breast cancer, which may result from decreased ratio of Bcl-2/Bax.

中图分类号:

R737.9

吴武军¹, 潘承恩¹, 曾健². 术前淋巴化疗对乳腺癌腋窝转移癌细胞Bcl-2和Bax蛋白表达的影响[J]. 南方医科大学学报, 2005, 25(08): 1001-1004.

WU Wu-jun¹, PAN Cheng-en¹, ZENG jian². Effect of preoperative lymphatic chemotherapy on Bcl-2 and Bax expression in axillary metastasis of breast cancer[J]. Journal of Southern Medical University, 2005, 25(08): 1001-1004.

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