南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (06): 751-752.

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补骨脂中补骨脂素的提取及纯化

刘志林1, 张相年2, 赵树进2, 周日笑3   

  1. 1. 广东外语外贸大学门诊部, 广东, 广州, 510420;
    2. 广州军区总医院药学部, 广东, 广州, 510010;
    3. 广东药学院药学系, 广东, 广州, 510240
  • 出版日期:2005-06-20 发布日期:2005-06-20
  • 基金资助:
    收稿日期:2004-12-8。
    作者简介:刘志林(1963- ),男,毕业于第二军医大学,主管药师,主要从事药房管理和药检工作,电话:020-36206728

Extraction and purification of psoralen from Psoralea corylifolia L.

LIU Zhi-lin1, ZHANG Xiang-nian2, ZHAO Shu-jin2, ZHOU Ri-xiao3   

  1. 1. 广东外语外贸大学门诊部, 广东, 广州, 510420;
    2. 广州军区总医院药学部, 广东, 广州, 510010;
    3. 广东药学院药学系, 广东, 广州, 510240
  • Online:2005-06-20 Published:2005-06-20

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摘要： 目的 从中药材补骨脂中提取补骨脂素。方法 补骨脂经粉碎后用50%乙醇浸渍提取,浸提液进行浓缩沉淀,得到膏状物,膏状物用甲醇溶解,活性碳脱杂质,再除去溶剂,放置过夜析出晶体。甲醇洗去稠状物得黄色片状晶体,甲醇重结晶后得针状白色晶体。薄层分析、高效液相色谱分析其纯度。紫外吸收光谱、H1-NMR谱鉴定其结构。结果与结论晶体物得率0.147%,是一个单体,经过波谱分析确定其为补骨脂素。用本方法获取纯品补骨脂素比较简便的方法。

Abstract: Objective To establish a method for extracting psoralen from the seed of Psoralea corylifolia L. Methods Crushed seed of Psoralea corylifolia L. was soaked with 50% ethyl alcohol solution, and the leaching solution was filtrated and distilled to remove alcohol. A slurry of the sediment was obtained and dissolved in methyl alcohol (MeOH), followed by treatment with active carbon for decoloration and evaporation of MeOH, resulting in a lamellar crystal after the sediment was kept still overnight. MeOH was applied to dilute the sticky solution, and after filteration, a yellow crystal was obtained, recrystallization of which in MeOH yielded white needle-like crystals. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were employed to determine the purity of the resultant substance, and UV and H1-NMR applied to identify its chemical structure. Results and Conclusion The resultant crystal is confirmed to be pure monomers of psoraren with a yield of 0.147% from the seed (m/m) by this simple extraction method.

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