外周血干细细移植后GVHD的动态监控可溶性人类白细胞抗原-I及临床意义

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (06): 687-690,695.

外周血干细细移植后GVHD的动态监控可溶性人类白细胞抗原-I及临床意义

夏荣^1,2, 邱慧颖¹, 章卫平¹, 曹琼², 兰炯采², 温宏升¹, 王健民¹

1. 第二军医大学长海医院血液科, 上海, 200433;
2. 南方医科大学南方医院输血科, 广东, 广州, 510515

出版日期:2005-06-20 发布日期:2005-06-20
基金资助:
收稿日期:2005-1-6。
基金项目:南方医科大学南方医院院长基金
作者简介:夏荣(1966- ),男,安徽芜湖人,博士,现为上海第二军医大学长海医院血液科临床医学博士后
通讯作者:王健民,电话:021-25070545,E-mail:jmwang@medmail.com.cn

Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection

XIA Rong^1,2, ¹, QIU Hui-ying¹, ZHANG Wei-ping², CAO Qiong², LAN Jiong-cai¹, WEN Hong-sheng¹

1. 第二军医大学长海医院血液科, 上海, 200433;
2. 南方医科大学南方医院输血科, 广东, 广州, 510515

Online:2005-06-20 Published:2005-06-20

摘要/Abstract

摘要： 目的研究动态监测可溶性人类白细胞抗原-I(sHLA-I)血清浓度对预测异基因外周血干细胞移植(allo-PBSCT)后移植物抗宿主病(GVHD)的意义。方法采用酶联免疫吸附试验(ELISA)双抗夹心法,以W6/32单克隆抗体包被酶标板,加被检血清,再加抗β2m辣根过氧化物酶及底物显色。测定63名中国汉族正常献血员sHLA-I含量,并对24例异基因外周血干细胞移植患者移植前及移植后不同时间段血清sHLA-I含量进行连续检测。结果 6例未发生GVHD和4例仅发生Ⅰ度GVHD移植受者sHLA-I水平在移植前后无明显变化(P>0.05);而发生Ⅱ-Ⅳ度GVHD的14例移植患者在发生GVHD前3~7d,sHLA-I浓度就有统计学意义的升高(P<0.05),在GVHD期升高程度更明显,具有显著性差异(P<0.005)。经免疫抑制剂冲击治疗至稳定期又降至基础水平。结论动态监控allo-PBSCT患者血清中sHLA-I含量对预测外周血干细胞移植后GVHD及治疗效果和预后有重要意义。

Abstract: Objective To assess the value of dynamic monitoring of soluble human lymphocytic antigen-I (sHLA-I) in the prediction of graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (PBSCT). Method Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantitatively detect serum sHLA-I. The serum samples for testing were added into W6 32 monoclonal antibody-coated microtiter plate and incubated with anti-β2m HRP followed by color development with the addition of the substrate. Serum sHLA-I level was measured in 63 healthy blood donors of Shanghai and in 24 PBSCT recipients before and and at different time points after the operation. Result No changes in sHLA-I levels occurred in allogeneic PBSCT recipients without GVHD or with only gradeⅠGVHD, but sHLA-I reached high levels in patients suffering GVHDⅡ-Ⅳ(P<0.05), which was effectively lowered by the application of immunosuppressants. Conclusion Measurements of sHLA-I levels can be valuable for monitoring GVHD after PBSCT.

中图分类号:

R329.24

夏荣^1,2, 邱慧颖¹, 章卫平¹, 曹琼², 兰炯采², 温宏升¹, 王健民¹. 外周血干细细移植后GVHD的动态监控可溶性人类白细胞抗原-I及临床意义[J]. 南方医科大学学报, 2005, 25(06): 687-690,695.

XIA Rong^1,2, ¹, QIU Hui-ying¹, ZHANG Wei-ping², CAO Qiong², LAN Jiong-cai¹, WEN Hong-sheng¹. Dynamic monitoring of graft-versus-host disease after allogeneic peripheral blood stem cell transplantation by soluble HLA-I antigen detection[J]. Journal of Southern Medical University, 2005, 25(06): 687-690,695.

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