蛋白质中变构通讯结构基础的方法分析与实现

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (06): 675-677,681.

蛋白质中变构通讯结构基础的方法分析与实现

谭小丹¹, 卢智勇², 苏永春¹, 董爱荣¹, 邓亲恺¹

1. 南方医科大学生物医学工程系, 广东, 广州, 510515;
2. 广东省机电职业技术学院, 广东, 广州, 510515

出版日期:2005-06-20 发布日期:2005-06-20
基金资助:
收稿日期:2004-10-13。
作者简介:谭小丹(1970- ),女,在读博士研究生
通讯作者:邓亲恺,教授,电话:020-61648279,E-mail:dqk001@fimmu.com

Analysis and realization of the method for predicting physical pathways of allosteric communi-cation in a protein family

TAN Xiao-dan¹, LU Zhi-yong², SU Yong-chun¹, DONG Ai-rong¹, DENG Qin-kai¹

1. 南方医科大学生物医学工程系, 广东, 广州, 510515;
2. 广东省机电职业技术学院, 广东, 广州, 510515

Online:2005-06-20 Published:2005-06-20

摘要/Abstract

摘要： 对变构通讯的理解是研究细胞信号转导的一个基本目标。本文介绍一种基于蛋白质家族序列的统计偶联分析方法,通过计算蛋白质序列中各氨基酸位点之间的统计偶联而获得相互偶联的氨基酸位点,构成变构通讯的结构基础。利用MATLAB语言编程实现了统计偶联分析方法;并首次根据2004年4月Swiss-Prot库中63395条真核蛋白质序列计算了各氨基酸在所有真核细胞蛋白序列中的频率分布平均值,这一结果与SteveW.Lockless等根据1998年10月Swiss-Prot库蛋白序列计算的结果基本一致。

Abstract: A fundamental goal in signal transduction study is to understand allosteric communication. The authors present hereby a statistical coupling analysis method (developed by Steve W. Lockless etc.) for quantitative mapping of the global network of amino acid positions in a protein and predicting a set of energetically coupled positions, which may constitute the physical pathways of allosteric communication in a protein family. Based on MATLAB, the authors realized this method and created histograms of amino acid distributions for all 63 395 entries (as of April 2004) in the Swiss-Prot database of eukaryotic proteins and calculated the mean values. The result was similar to that calculated by Steve W. Lockless in October 1998.

中图分类号:

谭小丹¹, 卢智勇², 苏永春¹, 董爱荣¹, 邓亲恺¹. 蛋白质中变构通讯结构基础的方法分析与实现[J]. 南方医科大学学报, 2005, 25(06): 675-677,681.

TAN Xiao-dan¹, LU Zhi-yong², SU Yong-chun¹, DONG Ai-rong¹, DENG Qin-kai¹. Analysis and realization of the method for predicting physical pathways of allosteric communi-cation in a protein family[J]. Journal of Southern Medical University, 2005, 25(06): 675-677,681.

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