常通口服液对术后肠粘连大鼠血清细胞因子的影响

摘要/Abstract

摘要： 目的观察常通口服液对术后粘连大鼠血液中肿瘤坏死因子α(TN F-α)、转化生长因子β₁(TG F-β1)、白细胞介素-1β、4、6、10(IL-1β、IL-4、IL-6、IL-10)水平的影响。方法选用SD 雄性大鼠,随机分为6 组:正常对照组、模型对照组、四磨汤组及常通口服液低、中、高剂量组。除正常对照组外,其余大鼠均按Ellis法制备成肠粘连模型。正常及模型对照组予以蒸馏水灌胃(10 ml/kg);四磨汤组以10 ml/kg 灌胃给药;常通口服液低、中、高剂量组分别按4.3、8.6 、17.2 g/kg 灌胃给药。各组于术后第7 天取血,酶联免疫吸附法测定细胞因子水平,同时进行粘连程度肉眼分级。结果常通口服液能显著减轻模型大鼠的肠粘连程度,降低术后肠粘连大鼠血清中致炎细胞因子TN F-α、IL-1β、TG F-β₁、IL-6 水平,但对抗炎细胞因子IL-4、IL-10 水平无影响。结论本实验初步筛选出有意义的术后粘连疗效评价指标,为术后粘连临床疗效评价和新药研发建立客观、准确、可靠的临床疗效评价指标提供了实验依据。

Abstract: Objective To observe the effects of Changtong oral liquid (CTOL) on the serum levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β₁, interleukin (IL)-1β, IL-4, IL-6 and IL-10 in rats with postoperative intestinal adhesions. Methods Fifty-four male Sprague-Dawley rats were randomized into 6 equal groups, namely the normal control, model, Simo decoction (SMD) groups and three CTOL groups of low, moderate, and high doses, respectively. Intestinal adhesion was induced in the rats of the groups other than the normal control group. The rats in the normal control and model groups received intragastric administration of distilled water (10 ml/kg), and those in the 4 treatment groups had SMD (10 ml/kg) and CTOL (at 4.3, 8.6 and 17.2 g/kg for low, moderate, and high dose groups, respectively). On day 7 after surgery, blood samples were obtained from the rats for measurement of serum cytokine levels with enzyme-linked immunosorbent assay followed by adhesion grading according to a 5-grade scale. Results CTOL evidently reduced the severity of postoperative adhesions and decreased the serum levels of the proinflammatory cytokines such as TNF-α, IL-1β, TGF-β₁ and IL-6. However, it had no significant impact on serum levels of the anti-inflammatory cytokines IL-4 and IL-10 in rats with postoperative adhesion. Conclusion Significant indices for postoperative adhesion assessment are established, which provides the experimental basis for evaluating clinical therapeutic effects of postoperative adhesions as well as for developing new therapeutic drugs.

中图分类号:

王春霞, 侯连兵, 刘世霆. 常通口服液对术后肠粘连大鼠血清细胞因子的影响[J]. 南方医科大学学报, 2005, 25(06): 601-604.

WANG Chun-xia, HOU Lian-bing, LIU Shi-ting. Effect of Changtong oral liquid on serum cytokine concentrations in rats with postoperative intestinal adhesion[J]. Journal of Southern Medical University, 2005, 25(06): 601-604.

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