南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (05): 567-569.

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TGF-β1和Ⅳ型胶原在慢性移植肾病患者肾组织中的表达及意义

韩聪祥1, 于立新1, 徐健1, 谢庆祥2, 王亦斌1, 邓文锋1, 刘小友1, 曾京华1   

  1. 1. 南方医科大学南方医院肾移植科, 广东, 广州, 510515;
    2. 解放军第175医院泌尿外科, 福建, 漳州, 363000
  • 出版日期:2005-05-20 发布日期:2005-05-20
  • 基金资助:
    收稿日期:2004-10-6。
    作者简介:韩聪祥(1975- ),男,在读硕士研究生,医师,电话:020-61641700,E-mail:xiangzi4318@sina.com

Expressions of transforming growth factor-β1 and collagen Ⅳ in renal tissues of patients with chronic allograft nephropathy

HAN Cong-xiang1, YU Li-xin1, XU Jian1, XIE Qing-xiang2, WANG Yi-bin1, DENG Wen-feng1, LIU Xiao-you1, ZENG Jing-hua1   

  1. 1. 南方医科大学南方医院肾移植科, 广东, 广州, 510515;
    2. 解放军第175医院泌尿外科, 福建, 漳州, 363000
  • Online:2005-05-20 Published:2005-05-20

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摘要： 目的 研究转化生长因子β1(TGF-β1)和Ⅳ型胶原在慢性移植肾病(CAN)患者肾组织中的表达及意义.方法 采用免疫组织化学技术及计算机图像分析系统,观测19例CAN患者移植肾组织中TGF-β1和Ⅳ型胶原的表达情况,分析两者间及与CAN病理分级之间的关系.结果 CAN移植肾组织中TGF-β1和Ⅳ型胶原的表达比正常肾组织明显增加(P<0.001),并随CAN病理分级呈逐渐递增的趋势;与正常肾组织相比TGF-β1和Ⅳ型胶原在CAN患者肾小球和肾小管间质中的表达呈正相关(r=0.94,P<0.001及r=0.910,P<0.001).结论 Ⅳ型胶原的异常沉积是CAN患者移植肾纤维化的重要表现,TGF-β1可能通过调控Ⅳ型胶原等成分的变化在CAN移植肾的纤维化进程中起重要作用.

Abstract: Objective To investigate the expressions of transforming growth factor (TGF)-β1 and collagen Ⅳ in the renal tissues of patients with chronic allograft nephropathy (CAN).Methods Immunohistochemical method and computer-assisted image analysis system were used to detect the expressions of TGF-β1 and collagen Ⅳ in the renal tissues of patients with CAN, and the association between TGF-β1 and collagen Ⅳexpressions as well as that between their expressions and the pathological grading of CAN were analyzed.Results The expressions of TGF-β1 and collagen Ⅳ were significantly higher in the renal tissues of the patients than in normal renal tissues (P<0.001), and the expressions tended to increase with the pathological grades of CAN; TGF-β1 and collagen Ⅳ expressions in both the renal glomeruli and the tubulointerstitium were in patients with CAN positively correlated with normal renal tissues (r=0.943, P<0.001; r=0.910, P<0.001).Conclusions Abnormal collagen Ⅳ deposition is one of the major factors associated with renal fibrosis in CAN, and TGF-β1 might play an important role in renal fibrosis in CAN through up-regulation of collagen Ⅳ in the renal tissues.

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