微囊藻毒素LR对小鼠的急性毒性研究

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (05): 565-566,572.

微囊藻毒素LR对小鼠的急性毒性研究

雷腊梅^1,2, 宋立荣¹

1. 中国科学院水生生物研究所, 湖北, 武汉, 430072;
2. 暨南大学水生生物研究中心, 广东, 广州, 510632

出版日期:2005-05-20 发布日期:2005-05-20
基金资助:
收稿日期:2004-11-5。
基金项目:国家重点基础研究发展规划项目(2002CB412306);中国科学院水生生物研究所创新领域前沿资助项目
作者简介:雷腊梅(1974- ),女,1996毕业于武汉大学,博士,电话:020-85220240,E-mail:amei_100@sina.com
通讯作者:宋立荣,电话:027-87217806;E-mail:lrsong@ihb.ac.cn

Acute toxicity of microcystin-LR in BALB/c mice

LEI La-mei^1,2, SONG Li-rong¹

1. 中国科学院水生生物研究所, 湖北, 武汉, 430072;
2. 暨南大学水生生物研究中心, 广东, 广州, 510632

Online:2005-05-20 Published:2005-05-20

摘要/Abstract

摘要： 目的了解微囊藻毒素对小鼠的急性损伤作用.方法以BALB/C小鼠为材料,腹腔注射μg./kg·b.w.和43μg/kg·b.w.剂量的微囊藻毒素LR,测定脏器系数和一系列生化指标.结果肾脏指数与肝脏指数明显增加,表明两种器官都受到损伤;血清中天冬氨酸转氨和丙氨酸转氨酶活力上升,尿素含量下降预示着肝脏损伤,尿酸含量上升则预示肾脏损伤的发生.结论肾脏也可能是易受微囊藻毒素损伤的器官.

Abstract: Objective To investigate acute toxicity of microcystin-LR in BALB/c mice.Methods BALB/c mice were subjected to intraperitoneal injection of microcystin-LR at the half lethal dose (LD₅₀) and 1/2 LD₅₀, and the organ weight indices and various biochemical indices were determined 30 min and 4 h after the treatment, respectively.Results Significant increase in liver or kidney weight index occurred in the treated mice, indicating hemorrhagic conditions of the two organs. A slight increase in serum levels of aspartate amino transferase and alanine aminotransferase was observed along with an obvious decrease in serum urea levels, suggesting liver damage. The increase of serum uric acid also indicated the presence of kidney damage.Conclusion The kidney may be another target of microcystins besides the liver.

中图分类号:

R123

雷腊梅^1,2, 宋立荣¹. 微囊藻毒素LR对小鼠的急性毒性研究[J]. 南方医科大学学报, 2005, 25(05): 565-566,572.

LEI La-mei^1,2, SONG Li-rong¹. Acute toxicity of microcystin-LR in BALB/c mice[J]. Journal of Southern Medical University, 2005, 25(05): 565-566,572.

参考文献

[1] HallegraffGM. A review of harmful algal bloom and their apparent global increase[J]. Phycologia, 1993, 32(2): 79-88.
[2] Yu SZ. Primary prevention ofhepatocellular carcinoma [J]. J Gastroenterol Hepatol, 1995, 10: 674-82.
[3] Rao PVL, Bhattacharya R, Oant SC, et al. Toxicity evaluation of in vitro cultures of fieshwater cyanobacterium Microcystis aeruginosa: I Hepatic and histopathological effects in rats [J]. Biomed Environ Sci, 1995, 8: 254-64.
[4] Gupta N, Pant SC, Vijayarghavan R, et al. Comparative toxicity evaluation of cyanobacterial cyclic peptide toxin microcystin variants (LR, RR, YR) in mice[J]. Toxicology, 2003, 188: 285-96.
[5] Hawkins PR, Chandrasena NR, Jones GJ. Isolation and toxicity of Cylindrospermopsis raciborskii fiom an ornamental lake [J].Toxicon, 1997, 35:341-6.
[6] Hooser SB, Beasley VR, Basgall EJ, et al. Microcystin-LR-induced ultrastmctural changes in rats[J]. Vet Pathol, 1990, 27(1): 9-15.
[7] 张青学,俞敏娟.铜绿微囊藻(Microcystis aeruginosa)水华毒性及毒素的研究[J].环境科学学报,1989,9:86-94.Zhang QX, Yu MJ. Studies on toxicity and toxins of Microcystis aeruginosa water-bloom[J]. J Environ Sci, 1989, 9: 86-94.
[8] Falconer IR, Stephen JH, Andrw RH. Hepatic and renal toxicity of the blue-green alga (cyanobacterium) Cylindrospermopsis reciborskii in male Swiss albino mice[J]. Environ Toxicol, 1999, 14: 143-50.
[9] Nishiwaki-Matsushima R, Ohta T, Nishiwaki S, et al. Liver tumor promotion by the cyanobacterial cyclic peptide toxin microcystin[J]. J Cancer Res Clin Oncol, 1992, 118(6): 420-4.
[10] Solter PF, Zonglin L, Guzman R. Decreased hepatic ALT synthesis is an outcome ofsub-chronic microcystin-LR toxicity [J]. Toxicol Appl Pharmaco1,2000, 164:216-20.
[11] Suchy FJ. Hepatocellular transport of bile acids [J]. Semin Liver Dis, 1993, 13(3): 235-47.
[12] Milutinovic A, Zivin M, Zorc-Pleskovic R, et al. Nephrotoxic effects of chronic administration of microcystins-LR and-YR [J]. Toxicon,2003, 42: 281-8.