中国蝮蛇蛇毒中小肽SV-PP-3的分离及其抗血小板聚集作用

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 476-478.

中国蝮蛇蛇毒中小肽SV-PP-3的分离及其抗血小板聚集作用

徐伟, 吴少瑜, 张嘉杰, 李志琴, 吴曙光

南方医科大学药物研究所, 广东, 广州, 510515

出版日期:2005-04-20 发布日期:2005-04-20
基金资助:
收稿日期:2005-1-5。
作者简介:徐伟(1971-),女,讲师,博士研究生,电话:020-61648167,E-mail:xuwei@fimmu.com
通讯作者:吴曙光,教授,博士生导师,电话:020-61648166

Purification of a small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus and its inhibition of platelet aggregation

XU Wei, WU Shao-yu, ZHANG Jia-jie, LI Zhi-qin, WU Shu-guang

南方医科大学药物研究所, 广东, 广州, 510515

Online:2005-04-20 Published:2005-04-20

摘要/Abstract

摘要： 目的研究中国蝮蛇蛇毒中具有抑制血小板聚集作用的小肽。方法采用低温离心、SephadexG-75凝胶层析、高效液相C-18反相层析从蛇毒中分离纯化小肽,并用血小板聚集仪测定分离的小肽在体外对磷酸腺苷(ADP)诱导的兔血小板聚集作用。结果 SV-PP-3是从中国蝮蛇蛇毒中分离出一种相对分子质量为1234.557的新的小肽。SV-PP-3在体外能显著抑制ADP诱导的兔血小板聚集,具有剂量依赖性。结论 SV-PP-3对ADP诱导的兔血小板聚集具有显著的抑制作用。

Abstract: Objective To investigate the inhibitory effect of the small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus on platelet aggregation. Methods The small peptide SV-PP-3 was isolated and purified by centrifugation at low temperature, sephadex G-75 column chromatography and reverse-phase (HPLC) on C-18 column. The platelet aggregation induced by adenosine diphosphate (ADP) was measured by LBY-NJ aggregometer. Results SV-PP-3 was purified from the venom of Agkistrodon blomhoffii siniticus, which inhibited the platelet ADP-induced platelet aggregation in a dose-dependent manner. Conclusion SV-PP-3 can inhibit ADP-Induced platelet aggregation.

中图分类号:

徐伟, 吴少瑜, 张嘉杰, 李志琴, 吴曙光. 中国蝮蛇蛇毒中小肽SV-PP-3的分离及其抗血小板聚集作用[J]. 南方医科大学学报, 2005, 25(04): 476-478.

XU Wei, WU Shao-yu, ZHANG Jia-jie, LI Zhi-qin, WU Shu-guang. Purification of a small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus and its inhibition of platelet aggregation[J]. Journal of Southern Medical University, 2005, 25(04): 476-478.

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