南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 454-456.

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空心莲子草抗登革病毒作用的实验研究

蒋文玲, 罗宪玲, 邝素娟   

  1. 广东省人民医院医学研究中心, 广东, 广州, 510080
  • 出版日期:2005-04-20 发布日期:2005-04-20
  • 基金资助:
    收稿日期:2004-9-11。
    作者简介:蒋文玲(1955-),女,副研究员,电话:020-83827812-42934,E-mail:jwenling@126.com

Effects of Alternanthera philoxeroides Griseb against dengue virus in vitro

JIANG Wen-ling, LUO Xian-ling, KUANG Su-juan   

  1. 广东省人民医院医学研究中心, 广东, 广州, 510080
  • Online:2005-04-20 Published:2005-04-20

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摘要： 目的 采用中药空心莲子草提取液进行体外抗登革病毒的研究,寻找新的抗登革病毒药物。方法 利用四甲基偶氮唑盐(MTT)法和细胞病变效应(CPE),观察检测空心莲子草对C6/36传代细胞株的细胞毒性作用和抗登革病毒作用。结果 空心莲子草的4种提取物除>320μg/ml组以外,各浓度组均未见对细胞有明显的毒性作用。用统计软件SPSS11.5的Probit回归法计算药物的半数毒性浓度(TD50)和半数有效抑制浓度(ED50),香豆精类提取物对细胞的毒性最小(TD50=535.91),石油醚提取物对病毒的抑制作用大于另3种提取物和药物对照(ED50=47.43)。结论 空心莲子草在体外有抗登革病毒作用。

Abstract: Objective To investigate the effects of Alternanthera philoxeroides Griseb extracts against dengue virus in vitro. Methods MTT assay and observation of cytopathic effect (CPE) were carried out to determine the cytotoxicity of Alternanthera philoxeroides Griseb on C6/36 cell lines and its effects on dengue virus. Results None of the 4 kinds of Alternanthera philoxeroides Griseb extracts exhibited obvious cytotoxicity on the cells at different concentrations with the exception of that over 320 μg/ml. The 4 extracts all showed inhibitory effects on dengue virus. Statistical analysis of TD50 and ED50 by Probit regression method suggested that extracts from coumarin had the lowest toxicity on the cells (TD50=535.91), whereas extracts from petroleum ether showed the strongest inhibitory effects on dengue virus (ED50=47.43) among the 4 extracts. Conclusion Alternanthera philoxeroides Griseb possesses antiviral effects on dengue virus in vitro.

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