南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 447-449,453.

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液质联用技术对复方蒲公英灌肠液的色谱指纹图谱的分析

晏媛1, 刘世霆1, 许重远1, 罗奇志2, 谭亚非1, 陈志良1   

  1. 1. 南方医科大学南方医院药学部, 广东, 广州, 510515;
    2. 南方医科大学中心实验室, 广东, 广州, 510515
  • 出版日期:2005-04-20 发布日期:2005-04-20
  • 基金资助:
    收稿日期:2004-9-13。
    作者简介:晏媛(1972-),女,现为南方医科大学在读硕士研究生,主管药师,研究方向:药物分析,电话:020-61641888-87215

High-performance liquid chromatography combined with mass spectrum analysis for chro-matographic fingerprinting of compound dandelion enema

YAN Yuan1, LIU Shi-ting1, XU Zhong-yuan1, LUO Qi-zhi2, TAN Ya-fei1, CHEN Zhi-liang1   

  1. 1. 南方医科大学南方医院药学部, 广东, 广州, 510515;
    2. 南方医科大学中心实验室, 广东, 广州, 510515
  • Online:2005-04-20 Published:2005-04-20

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摘要： 目的 确定复方蒲公英灌肠液中的活性成分。方法 利用三维高效液相色谱对复方蒲公英灌肠液的乙酸乙酯部分分离分析,采用液相色谱‐质谱联用技术对其色谱图谱进行确定。结果 通过质谱的质荷比(M/Z)及色谱保留时间的相互比较,确定了咖啡酸、阿魏酸和原儿茶醛3种活性成分。结论 液质联用技术可用于复方蒲公英灌肠液的定性分析。

Abstract: Objective To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases. Methods Three-dimensional high-performance liquid chroma- tography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting. Result By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde. Conclusion HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.

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