南方医科大学学报

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 413-415.

• • 上一篇    下一篇

丝裂霉素C聚氰基丙烯酸正丁酯磁性纳米球血药浓度的测定

刘炜, 陈建海, 任非, 郭云波, 阎玺庆, 刘焰东   

  1. 南方医科大学南方医院药学部, 广东, 广州, 510515
  • 出版日期:2005-04-20 发布日期:2005-04-20
  • 基金资助:
    收稿日期:2004-10-18。
    基金项目:国家863项目(2002AA214121);国家自然科学基金项目(30270400)
    作者简介:刘炜(1973-),男,黑龙江哈尔滨市人,在读硕士研究生,020-61641888-87236,Email:lw0913@fimmu.com
    通讯作者:陈建海,教授,博士生导师,电话:020-61642173

Determination of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma

LIU Wei, CHEN Jian-hai, REN Fei, GUO Yun-bo, YAN Xi-qing, LIU Yan-dong   

  1. 南方医科大学南方医院药学部, 广东, 广州, 510515
  • Online:2005-04-20 Published:2005-04-20

PDF

972

摘要： 目的 建立高效液相色谱法测定小鼠血浆中丝裂霉素C聚氰基丙烯酸正丁酯磁性纳米球浓度的方法。方法 血浆样品经乙酸乙酯提取后,以乙腈∶醋酸钠缓冲液pH(15∶85)为流动相,使用LiChroCART LiChrospher RP-18(250mm×4mm,5 μm)色谱柱分离;流速为1.0ml/min;检测波长为365nm。结果 血浆中丝裂霉素C测定的线性范围为0.04~1.00 μg/ml,线性方程为:Y=16388X-17.17, r=0.9998。结论 该方法简单实用,结果准确,适用于丝裂霉素C-聚氰基丙烯酸正丁酯磁性纳米制剂临床前药代动力学研究。

Abstract: Objective To establish a method by high-performance liquid chromatography (HPLC) for determining the concentration of magnetic mitomycin C-polybutylcyanoacrylate nanoparticles in mouse plasma. Methods Chromatography was performed on a LiChroCART C18 (250 mm×4 mm, 5 μm) column with the mobile phase consisting of acetonitrile-NaAC (15:85), the flow rate of 1.0 ml/min, and the detection wavelength of 365 nm. Sample extraction was carried out with ethylacetate. Results The linear range of mouse plasma mitomycin C concentration was 0.04-1.00 μg/ml, and the linear equation of Y=16 388X-17.17 (r=0.999 8) was derived. Conclusion This method is very easy to operate and suits the need of perclinical pharmacokinetic studies of mitomycin-magnetic nanoparticles and yields accurate and precise results.

中图分类号: