鸸鹋油对烫伤大鼠创面的抗炎及促愈合作用

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 407-410.

鸸鹋油对烫伤大鼠创面的抗炎及促愈合作用

邱学文¹, 王甲汉¹, 方小文², 龚震宇¹, 李志清¹, 易朝辉¹

1. 南方医科大学南方医院烧伤科, 广东, 广州, 510515;
2. 广州英吉利实业有限公司, 广东, 广州, 510515

出版日期:2005-04-20 发布日期:2005-04-20
基金资助:
收稿日期:2004-7-3。
基金项目:广东省自然科学基金重点项目(020089)
作者简介:邱学文(1978-),男,2004年毕业于第一军医大学,硕士,电话:020-61641888-87250,E-mail:shavin@sohu.com

Anti-inframnatory activity and healing-promoting effects of topical application of emu oil on wound in scalded rats

QIU Xue-wen¹, WANG Jia-han¹, FANG Xiao-wen², GONG Zhen-yu¹, LI Zhi-qing¹, YI Zhao-hui¹

1. 南方医科大学南方医院烧伤科, 广东, 广州, 510515;
2. 广州英吉利实业有限公司, 广东, 广州, 510515

Online:2005-04-20 Published:2005-04-20

摘要/Abstract

摘要： 目的从炎症反应的角度探讨外用鸸鹋油对烫伤大鼠创面愈合的影响。方法建立10%体表面积浅Ⅱ度烫伤大鼠模型,将144只雄性Wistar大鼠随机分为A、B、C组,分别外用生理盐水、碘伏、鸸鹋油治疗,观察创面的大体变化及愈合情况,分别留取不同时相的创面和血液标本,进行组织病理学检查和含水量测定(干湿重法)、ELISA法检测组织和血浆TNFα含量。结果外用鸸鹋油可使创面肿胀、渗出减轻,创面无感染迹象,未见任何副作用;病理形态学观察发现鸸鹋油可使局部组织炎症反应减轻,与A、B组相比较,以伤后1、3d差异最为明显;C组伤后3d组织含水量及TNF-α含量明显减少(P<0.01),两者之间存在正相关关系(P<0.001);可使创面愈合时间缩短(P<0.01),促进创面再上皮化和表皮各层的分化。结论在浅Ⅱ度烫伤创面外用鸸鹋油具有抗炎作用,可能与局部组织中促炎细胞因子水平的降低有关;并能促进创面愈合,可能与其抑制局部组织的继发性炎症反应有关。

Abstract: Objective To investigate the effects of topical application of emu oil on wound healing in scalded rats. Methods In 144 male Wistar rats with 10% total body surface superficial Ⅱ degree scald treated on a random basis with physiological saline, povidone iodine and emu oil, respectively, the changes of the wound were observed and the wound tissue and blood samples harvested at different times after injury for evaluation of histopathological changes, total tissue water content (measured by wet:dry weight ratios), and tumor necrosis factor (TNF)-α levels in the wound tissue and plasma by enzyme-linked immunosorbent assay (ELISA). The general condition of the wound healing was also observed. Results After application of emu oil, the swelling and effusion of the burn wound were alleviated and evidences of wound infection or adverse effects were not observed. Pathological examination showed that emu oil could alleviate topical inflammation, which was particularly obvious on days 1 and 3 after injury as compared with the other two groups. On day 3 after injury, water content and TNF-α level in the tissues was markedly decreased with the application of emu oil (P<0.05), with a significant correlation between their changes (P<0.001) and shortened wound healing time (P<0.05). Pathological examination showed that emu oil could promote epithelialization and differentiation of various epidermal layers. Conclusion Emu oil has topical anti-inflammatory activity in rats with superficial Ⅱ degree scald, possibly in association with decreased levels of the proinflammatory cytokines in the tissues and can promote wound healing by inhibiting local secondary inflammation.

中图分类号:

R644

邱学文¹, 王甲汉¹, 方小文², 龚震宇¹, 李志清¹, 易朝辉¹. 鸸鹋油对烫伤大鼠创面的抗炎及促愈合作用[J]. 南方医科大学学报, 2005, 25(04): 407-410.

QIU Xue-wen¹, WANG Jia-han¹, FANG Xiao-wen², GONG Zhen-yu¹, LI Zhi-qing¹, YI Zhao-hui¹. Anti-inframnatory activity and healing-promoting effects of topical application of emu oil on wound in scalded rats[J]. Journal of Southern Medical University, 2005, 25(04): 407-410.

参考文献

[1] 常永宏,朱周财,车锡平,等.鸸鹋油的抗炎镇痛作用[J].西北药学杂志(Northwest Pharm J),1998,13(6):256.
[2] Lopez A, Sims DE, Ablett RF, et al. Effect of emu oil on auricular inflammation induced with croton oil in mice[J]. Am J Vet Res,1999, 60(12): 1558-61.
[3] Yoganathan S, Nicolosi R, Wilson T, et al. Antagonism of croton oil inflammation by topical emu oil in CD-1 mice[J]. Lipids, 2003, 38(6): 603-7.
[4] 李志清,王甲汉,任加良,等.鸸鹋油对烫伤大鼠创面的愈合作用[J].第一军医大学学报,2004,24(11):1255-6Li ZQ, Wang JH, Ren JL, et al. Effects of topical emu oil on wound healing in scalded rats[J]. J First Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2004, 24(11): 1255-6.
[5] Snowden JM, Whitehouse MW. Anti-inflammatory activity of emu oil in rats[J]. Inflammopharmacology, 1997, 5(2): 127-32.
[6] Whitehouse MW, Turner AG, Davis CKC, et al. Emu Oil(s): A source of non-toxic transdermal anti-inflammatory agents in aboriginal medicine[J]. Inflammopharmacology, 1998, 6(1): 1-8.
[7] Politis MJ, Dmytrowich A. Promotion of second intention wound healing by emu oil lotion: comparative results with furasin, polysporin,and cortisone[J]. Plast Reconstr Surg, 1998, 102(7): 2404-7.
[8] Nair VB, Panchagnula R. Effect of iontophoresis and fatty acids on permeation of arginine vasopressin through rat skin[J]. Pharmacol Res, 2003, 47(6): 563-9.
[9] Jiang SJ, Zhou XJ. Examination of the mechanism ofoleic acidinduced percutaneous penetration enhancement: an ultra structural study[J]. Biol Pharm Bull, 2003, 26(1): 66-8.
[10] 朱玉云,李娟,高允华.复方碘伏片及其主要成分的抗炎作用研究[J].泰山医学院学报,2000,21(3):192-4.Zhu YY, Li J, Gao YS, et al. The anti-inflammatory effects of compound polyvidone iodine tablet and its active components in animal models[J]. J Taishan Med Coll, 2000, 21(3): 192-4.
[11] Ribeiro Barros Cardoso C, Aparecida Souza M, Amalia Vieira Ferro E, et al. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds[J]. Wound Repair Regener, 2004, 12(2): 235-43.
[12] Hinton A Jr, Ingram KD. Use of oleic acid to reduce the population of bacterial flora of poultry skin[J]. J Food Prot, 2000, 63(9): 1282-6.
[13] YffBT, Lindsey KL, Taylor MB, et al. The pharmacological screening of Pentanisia prunelloides and the isolation of the antibacterial compound palmitic acid[J]. J Ethnopharmacol, 2002,79(1): 101-7.