新基因LX3与白细胞介素-6诱导的相关性

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 384-386.

新基因LX3与白细胞介素-6诱导的相关性

夏荣^1,2, 黎燕², 冯建男², 兰炯采¹, 沈倍奋²

1. 南方医科大学南方医院输血科, 广东, 广州, 510515;
2. 军事医学科学院基础医学研究所, 北京, 100850

出版日期:2005-04-20 发布日期:2005-04-20
基金资助:
收稿日期:2004-12-6。
基金项目:国家杰出青年科学基金(39925019)
作者简介:夏荣(1966-),男,安徽芜湖人,博士,主治医师,电话020-61641038
通讯作者:沈倍奋

Association of interleukin-6 induction and the novel gene LX3

XIA Rong^1,2, LI Yan², FENG Jian-nan², LAN Jiong-cai¹, SHEN Bei-fen²

1. 南方医科大学南方医院输血科, 广东, 广州, 510515;
2. 军事医学科学院基础医学研究所, 北京, 100850

Online:2005-04-20 Published:2005-04-20

摘要/Abstract

摘要： 目的研究功能未知新基因LX3^[1]与白细胞介素-6(IL-6)的相关性,为研究IL-6的作用机制寻找新的靶基因。方法反转录-PCR(RT-PCR)分析不同浓度IL-6处理的U937细胞及同一浓度IL-6诱导不同时间的U937细胞中新基因LX3的表达差异;NorthernBlot分析IL-6诱导前后U937细胞中新基因LX3表达的变化。结果 LX3基因在U937细胞中的表达量随IL-6诱导浓度的增加而增加,在IL-6浓度为500ng/ml时表达量最高,而在0ng/ml时不表达;时间表达谱分析显示在IL-6刺激8h时新基因LX3的表达量最高;Northern印迹分析显示新基因LX3在IL-6诱导后的U937细胞中的表达量明显升高。结论 LX3是与IL-6诱导紧密相关的新基因。

Abstract: Objective To analyze the association between the expression of the novel gene LX3 and interleukin-6 (IL-6) induction, and explore a new target gene for action mechanism of IL-6. Methods The total RNA was extracted from U937 cells induced by IL-6 at different concentrations and varied lengths of time. Reverse transcriptional (RT)-PCR and Northern blotting were employed to determine the expression of LX3 and IL-6 induction. Results The expression of novel gene LX3 was increased while concentration of IL-6 was improved gradually. High expression was induced by IL-6, which was highest at the concentration was 500 ng/ml and no expression at 0 ng/ml. Time- expression pattern proved that expression of novel gene LX3 of higest at 8 hour after induced by IL-6. Northern blotting confirmed that the expression quantity of LX3 increased in U937 cells induced by IL-6. Conclusion The quantity of LX3 expression is associated with the dose and duration of IL-6 induction, suggesting that LX3 gene is a novel gene correlated with IL-6 induction.

中图分类号:

夏荣^1,2, 黎燕², 冯建男², 兰炯采¹, 沈倍奋². 新基因LX3与白细胞介素-6诱导的相关性[J]. 南方医科大学学报, 2005, 25(04): 384-386.

XIA Rong^1,2, LI Yan², FENG Jian-nan², LAN Jiong-cai¹, SHEN Bei-fen². Association of interleukin-6 induction and the novel gene LX3[J]. Journal of Southern Medical University, 2005, 25(04): 384-386.

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