广州管圆线虫Mr32000抗原的免疫诊断效果评价

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (04): 380-383.

广州管圆线虫M_r32000抗原的免疫诊断效果评价

李华¹, 陈晓光¹, 沈浩贤², 陈代雄², 裘玉蓉³, 胡小佳³

1. 南方医科大学, 寄生虫学教研室, 广东, 广州, 510515;
2. 广州医学院病原生物学教研室, 广东, 广州, 510182;
3. 南方医院检验科, 广东, 广州, 510515

出版日期:2005-04-20 发布日期:2005-04-20
基金资助:
收稿日期:2004-11-6。
基金项目:国家科技部十五重大科技攻关项目(2003BA712A03-07)
作者简介:李华(1968-),男,在读博士,电话:020-61640114-89122
通讯作者:陈晓光,教授,南方医科大学寄生虫学教研室,电话:020-61648308

Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis

LI Hua¹, CHEN Xiao-guang¹, SHEN Hao-xian², CHEN Dai-xiong², QIU Yu-rong³, HU Xiao-jia³

1. 南方医科大学, 寄生虫学教研室, 广东, 广州, 510515;
2. 广州医学院病原生物学教研室, 广东, 广州, 510182;
3. 南方医院检验科, 广东, 广州, 510515

Online:2005-04-20 Published:2005-04-20

摘要/Abstract

摘要： 目的评价广州管圆线虫Mr32000抗原(AC32)的诊断价值。方法利用切胶纯化法从广州管圆线虫成虫抗原中获取AC32,用Westernblotting和ELISA方法检测61例广州管圆线虫感染大鼠血清、5例正常鼠血清、1例广州管圆线虫病人血清、50例其他寄生虫体阳性患者血清和50例献血员血清。结果 AC32和成虫粗抗原包被的ELISA检测61份感染大鼠血清和1例临床诊断为广州管圆线虫病人血清均为阳性;AC32-ELISA检测的50例献血员、20例急性血吸虫病人、11例弓形虫病人和所检测的其他寄生虫抗体阳性患者血清均为阴性。结论 AC32在广州管圆线虫病的血清学诊断中具有较高的敏感性和特异性。

Abstract: Objective To evaluate the specificity and sensitivity of immunodiagnosis with the antigen with molecular mass of 32 000 of Angiostrongyliasis cantonensis(AC32). Methods The major antigenic protein AC32 was purified from the antigens of A.cantonensis adult worm by electroelution from SDS-polyacrylamide gels. AC32-enzyme linked immunosorbent assay (ELISA) and adult worm antigen (AWA)-ELISA were used to detect the specific IgG in the sera of normal rats (n=5) and rats with angiostrongyliasis (n=61), sera of healthy individuals (n=50) and patients (n=50) with angiostrongyliasis, schistosomiasis and otherparasitosis. Results The positivity rate in AC32-ELISA of the sera from rats with angiostrongyliasis was 100% without false positive results or cross reaction between AC32 and the sera of the normal control and patients infected with parasites other than A.cantonensis. Conclusion AC32 of A.cantonensis is a valuable candidate antigen for immunodiagnosis of angiostrongyliasis with high sensitivity and specificity.

中图分类号:

李华¹, 陈晓光¹, 沈浩贤², 陈代雄², 裘玉蓉³, 胡小佳³. 广州管圆线虫M_r32000抗原的免疫诊断效果评价[J]. 南方医科大学学报, 2005, 25(04): 380-383.

LI Hua¹, CHEN Xiao-guang¹, SHEN Hao-xian², CHEN Dai-xiong², QIU Yu-rong³, HU Xiao-jia³. Value of the antigen with molecular mass of 32 000 in immunodiagnosis of Angiostrongylus cantonensis[J]. Journal of Southern Medical University, 2005, 25(04): 380-383.

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