糖基化终极产物对乳鼠心肌细胞胞内游离钙瞬间变化的影响

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (03): 274-276,280.

糖基化终极产物对乳鼠心肌细胞胞内游离钙瞬间变化的影响

邵亚辉¹, 许顶立¹, 曾平¹, 赖文岩², 任昊¹, 江荣炎¹

1. 南方医科大学南方医院心血管内科, 广东, 广州, 510515;
2. 南方医科大学南方医院肾内科, 广东, 广州, 510515

出版日期:2005-03-20 发布日期:2005-03-20
基金资助:
收稿日期:2004-10-11。
基金项目:国家重点基础研究发展规划(G2000056905);广东省自然科学基金研究团队项目(10717)
作者简介:邵亚辉(1971- ),女,1994年毕业于山东医科大学,南方医科大学在读硕士,主治医师,联系电话:020-61365855,E-mail:cinderel@fimmu.com.
通讯作者:许顶立,教授,主任医师

Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment

SHAO Ya-hui¹, XU Ding-li¹, ZENG Ping¹, LAI Wen-yan², REN Hao¹, JIANG Rong-yan¹

1. 南方医科大学南方医院心血管内科, 广东, 广州, 510515;
2. 南方医科大学南方医院肾内科, 广东, 广州, 510515

Online:2005-03-20 Published:2005-03-20

摘要/Abstract

摘要： 目的探讨糖基化终极产物(AGEs)刺激对体外培养乳鼠心肌细胞内游离钙的影响。方法应用荧光钙离子指示剂Fluo-3/AM将体外培养3~5 d的乳鼠心肌细胞染色,激光共聚焦显微镜(LSCM)观察不同浓度AGEs(50、100、200 μg/ml)对心肌细胞瞬时刺激后细胞内游离钙离子浓度的影响。结果与对照组相比,AGEs刺激可以迅速引起心肌细胞胞内游离钙浓度的升高,其恢复时程显著延长并呈剂量依赖性。结论 AGEs可能通过改变心肌细胞内游离钙离子浓度对心肌细胞造成损伤。

Abstract: Objective To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.Methods CMs cultured for 3 to 5 days in vitro were incubated with Ca²⁺-sensitive fluorescent indicator Fluo-3AM with light screening at 37℃ with 5% CO₂ for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).Results Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.Conclusion AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration.

中图分类号:

邵亚辉¹, 许顶立¹, 曾平¹, 赖文岩², 任昊¹, 江荣炎¹. 糖基化终极产物对乳鼠心肌细胞胞内游离钙瞬间变化的影响[J]. 南方医科大学学报, 2005, 25(03): 274-276,280.

SHAO Ya-hui¹, XU Ding-li¹, ZENG Ping¹, LAI Wen-yan², REN Hao¹, JIANG Rong-yan¹. Transient cytosolic free calcium changes in cultured neonatal rat cardiac myocytes in response to advanced glycation end-product treatment[J]. Journal of Southern Medical University, 2005, 25(03): 274-276,280.

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