低G预适应对高G暴露致大鼠学习记忆功能受损的影响

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (02): 212-215.

低G预适应对高G暴露致大鼠学习记忆功能受损的影响

曹新生, 吴兴裕, 孙喜庆, 刘挺松

第四军医大学航空航天医学系航动教研室, 陕西, 西安, 710032

出版日期:2005-02-20 发布日期:2005-02-20
基金资助:
收稿日期:2004-8-12。
作者简介:曹新生(1974- ),男,2004年毕业于第四军医大学,博士,讲师,电话:029-3374813,E-mail:caoxinsheng@163.net

Effects of low gravity preconditioning on rat learning and memory impairment induced by high gravity exposure

CAO Xin-sheng, WU Xing-yu, SUN Xi-qing, LIU Ting-song

第四军医大学航空航天医学系航动教研室, 陕西, 西安, 710032

Online:2005-02-20 Published:2005-02-20

摘要/Abstract

摘要： 目的探讨低G预适应对高G致大鼠学习记忆功能受损的影响。方法 24只雄性SD大鼠随机分为对照组、+10Gz/3min组和低G预适应组,每组8只。低G预适应组在+10Gz/3min暴露前进行3d的+4Gz/3min锻炼(1次/d)。观察单次+10Gz/3min暴露后0、2、4和6d大鼠学习记忆功能的变化。结果旷场反应中,+10Gz/3min组中央格停留时间在0d,2d较对照组显著延长(P<0.05),总得分在0d显著降低(P<0.01);低G预适应组0d的中央格停留时间显著延长(P<0.01),总得分显著降低(P<0.01)。与+10Gz/3min组比较,低G预适应组中央格停留时间从2d开始有缩短趋势,6d时达到显著水平(P<0.05),各时间点总得分均无显著差异。辨别性学习能力测试中,+10Gz/3min组正确反应次数在各时间点均较对照组显著下降,反应时在各时间点均较对照组显著延长(P<0.01);低G预适应组正确反应次数和反应时在各时间点均与对照组无明显差别;与+10Gz/3min组比较,低G预适应组在各时间点的正确反应次数均显著增加(P<0.01),反应时均显著缩短(P<0.01)。被动回避反应中,与对照组比较,+10Gz/3min组的0d潜伏期显著延长(P<0.05),6d潜伏期显著缩短(P<0.01),学习所用总时间与错误数无显著性变化;低G预适应组各项检测项目均无明显改变;与+10Gz/3min组比较,低G预适应组大鼠的6d潜伏期显著延长(P<0.01)。结论采用低G预适应措施后,能够显著改善高G导致的大鼠学习和记忆能力受损程度。

Abstract: Objective To study the protective effects of low gravity preconditioning against learning and memory function impairment induced by high gravity exposure in rats. Method Twenty-four male SD rats were randomized equally into control group, high gravity exposure group(+10 Gz/3 min) and low gravity preconditioning group(LGP group, preconditioned by a daily exposure to +4 Gz/3 min for 3 days before +10 Gz/3 min exposure). Both the learning and memory abilities of the rats in the 3 groups were examined after +10 Gz/3 min exposure. Results In open field test, the rats exposed to +10 Gz/3 min, in comparison with the control rats, exhibited significantly declined total square-crossing and rearing(SCR) on days 0 and 2(P<0.05), with also significantly prolonged stay in the central square at 0 d(P<0.01). In the low gravity preconditioned rats, SCR declined and length of stay in the central square increased significantly on day 0(P<0.01). Compared with the rats in +10 Gz/3 min group, the rats in the LGP group showed decreased length of stay in the central square on day 2, and such changes was statistically significant on day 6(P<0.05), but there was no difference in SCR at all time points. In Y-maze test, the number of times of right reaction(RR) decreased and the reaction time(RT) increased significantly in +10 Gz/3min group compared with those of the control group(P<0.01), whereas no significant differences were found between the control group and LGP group. Compared with +10 Gz/3 min group, the RR was increased(P<0.01) and RT shortened significantly(P<0.01) in LGP group. In the step-through test, the latent time(LT) increased(P<0.05) on day 0 but was then shortened significantly on day 6 in + 10 Gz/3min group(P<0.01), and no difference was found between LGP group and the control group. LT on day 6 increased significantly(P<0.01) in LGP group as compared with the +10 Gz/3 min group. Conclusion High gravity exposure-induced rat learning and memory impairment can be markedly improved by lower gravity preconditioning.

中图分类号:

R852.21

曹新生, 吴兴裕, 孙喜庆, 刘挺松. 低G预适应对高G暴露致大鼠学习记忆功能受损的影响[J]. 南方医科大学学报, 2005, 25(02): 212-215.

CAO Xin-sheng, WU Xing-yu, SUN Xi-qing, LIU Ting-song. Effects of low gravity preconditioning on rat learning and memory impairment induced by high gravity exposure[J]. Journal of Southern Medical University, 2005, 25(02): 212-215.

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