南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (02): 198-200.

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32P照射对HL-60细胞增殖周期的影响

何子毅, 孟庆勇   

  1. 广东医学院分析中心, 广东, 湛江, 524023
  • 出版日期:2005-02-20 发布日期:2005-02-20
  • 基金资助:
    收稿日期:2004-8-15。
    基金项目:广东省卫生厅科学基金(A2002466)
    作者简介:何子毅(1974- ),男,硕士研究生,主要从事低辐射生物效应的研究

Effect of 32P irradiation on cell cycle of HL-60 cells

HE Zi-yi, MENG Qin-yong   

  1. 广东医学院分析中心, 广东, 湛江, 524023
  • Online:2005-02-20 Published:2005-02-20

摘要: 目的 观察32P照射对HL-60细胞周期的影响。方法 不同剂量32P体外照射HL-60细胞24、48和72h,流式细胞法检测细胞周期。结果 不同剂量32P照射HL-60细胞对细胞周期有明显影响,首先发生G2/M期阻滞;随剂量增大,G2/M期和S期均发生阻滞,以G2/M期阻滞为主。结论 32P体外照射HL-60细胞对细胞周期有明显的干扰作用。

Abstract: Objective To investigate the effect of 32P irradiation on the cell cycle of HL-60 cells. Methods Flow cytometry was employed to detect cell cycle changes of HL-60 cells induced by 32P irradiation at different doses for varied time lengths(24, 28 and 72 h, respectively). Result 32P could induce cell cycle arrest in S and G2/M phases, and the arrest occurred initially in G2/M phase. Conclusion 32P may significantly interfere with the cell cycle of HL-60 cells, causing cell cycle arrest primarily in G2/M.

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