侧脑室注射链脲霉素引起大鼠脑Aβ1-40、Aβ1-42和tau202、tau396、tau404表达增加

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (02): 168-170,173.

侧脑室注射链脲霉素引起大鼠脑Aβ_1-40、Aβ_1-42和tau²⁰²、tau³⁹⁶、tau⁴⁰⁴表达增加

褚文政, 钱采韻

中山大学附属第一医院神经科, 广东, 广州, 510080

出版日期:2005-02-20 发布日期:2005-02-20
基金资助:
收稿日期:2004-11-6。
基金项目:国家自然科学基金(39370264);“九五”国家医学科技攻关项目(96-906-05-07)
作者简介:褚文政(1971- ),男,在读博士研究生,主治医师,E-mail:bzchu@yahoo.com..cn
通讯作者:钱采韻(1940- ),女,教授,博士生导师,主要研究方向:缺血性脑血管病和Alzheimer病,E-mail:qiancy@vip.163.com

Expressions of Aβ_1-40, Aβ_1-42, tau²⁰², tau³⁹⁶ and tau⁴⁰⁴ after intracerebroventricular injection of streptozotocin in rats

CHU Wen-zheng, QIAN Cai-yun

中山大学附属第一医院神经科, 广东, 广州, 510080

Online:2005-02-20 Published:2005-02-20

摘要/Abstract

摘要： 目的探讨用链脲霉素(Streptozotocin,STZ)阻断脑内胰岛素信号传导,引起脑能量代谢障碍与Aβ表达及tau蛋白过度磷酸化的关系。方法 24只SD大鼠随机分为实验组和生理盐水组,实验组双侧侧脑室注入STZ3mg/kg,第3天重复此剂量;生理盐水组手术操作相同,但注入等量的生理盐水代替STZ。21d后,采用免疫组化方法观测各组大鼠大脑皮层和海马区的Aβ_1-40、Aβ_1-42、tau³⁹⁶、tau⁴⁰⁴阳性细胞表达。结果与生理盐水组相比,实验组皮层和海马Aβ_1-40、Aβ_1-42、tau²⁰²、tau³⁹⁶、tau⁴⁰⁴阳性细胞明显增多。结论侧脑室注射STZ可以引起脑内Aβ表达增多和tau蛋白过度磷酸化。

Abstract: Objective To investigate the effects of intracerebroventricular(ICV) administration of streptozotocin(STZ) on the expressions of Aβ and hyperphosphorylation of tau protein in rat brain. Methods Twenty-four adult SD rats were randomized into 2 groups to receive ICV of STZ bilaterally at the dose of 3 mg/kg(with the injection repeated on day 3) or normal saline injection in an identical manner. Twenty-one days later, the expressions of Aβ_1-40,Aβ_1-42, tau²⁰², tau³⁹⁶ and tau⁴⁰⁴ were investigated immunohistochemically. Results After STZ administration, the expressions of Aβ_1-40,Aβ_1-42, tau²⁰², tau³⁹⁶ and tau⁴⁰⁴ increased in both the cortex and hippocampus in the rat brain. Conclusion ICV injection of STZ increases the expression of Aβand promotes hyerphosphorylation of tau protein.

中图分类号:

R749.1

褚文政, 钱采韻. 侧脑室注射链脲霉素引起大鼠脑Aβ_1-40、Aβ_1-42和tau²⁰²、tau³⁹⁶、tau⁴⁰⁴表达增加[J]. 南方医科大学学报, 2005, 25(02): 168-170,173.

CHU Wen-zheng, QIAN Cai-yun. Expressions of Aβ_1-40, Aβ_1-42, tau²⁰², tau³⁹⁶ and tau⁴⁰⁴ after intracerebroventricular injection of streptozotocin in rats[J]. Journal of Southern Medical University, 2005, 25(02): 168-170,173.

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侧脑室注射链脲霉素引起大鼠脑Aβ_1-40、Aβ_1-42和tau²⁰²、tau³⁹⁶、tau⁴⁰⁴表达增加

Expressions of Aβ_1-40, Aβ_1-42, tau²⁰², tau³⁹⁶ and tau⁴⁰⁴ after intracerebroventricular injection of streptozotocin in rats

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