人脐血单个核细胞诱导分化为神经元样细胞

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (02): 152-155.

人脐血单个核细胞诱导分化为神经元样细胞

柳太云¹, 张成¹, 肖露露², 姚晓黎¹, 冯善伟¹, 曾缨¹

1. 中山大学附属第一医院神经内科, 广东, 广州, 510089;
2. 广州血液中心干细胞室, 广东, 广州, 510095

出版日期:2005-02-20 发布日期:2005-02-20
基金资助:
收稿日期:2004-10-15。
基金项目:卫生部临床学科重点项目(2001321);国家自然科学基金(30170337)
作者简介:柳太云(1964- ),男,副主任医师,在读博士研究生,从事神经遗传性疾病研究,电话:020-87334192,E-mail:ltylxlf@gzsums.edu.cn
通讯作者:张成,电话:020-86332387;E-mail:czym@gzsums.edu.cn

Induced differentiation of human cord blood monocytes into neuron-like cells in vitro

LIU Tai-yun¹, ZHANG Cheng¹, XIAO Lu-lu², YAO Xiao-li¹, FENG Shan-wei¹, ZENG Ying¹

1. 中山大学附属第一医院神经内科, 广东, 广州, 510089;
2. 广州血液中心干细胞室, 广东, 广州, 510095

Online:2005-02-20 Published:2005-02-20

摘要/Abstract

摘要： 目的探讨人脐血单个核细胞体外向神经元样细胞定向诱导分化的条件。方法无菌条件下采集正常人脐血,分离单个核细胞。用DMEM/F12培养基进行纯化和扩增培养。取扩增5代的间充质干细胞(MSC),分别用β-巯基乙醇(β-ME)、二甲基亚砜(DMSO)、神经条件培养液诱导脐血MSC向神经元样细胞分化。免疫组化法检测脐血MSC的Nestin表达和神经元样细胞特异性标志。结果脐血原代和2、5代MSC的Nestin表达阳性率分别为6.7%、12.4%和20.8%。神经条件培养液诱导神经元样细胞阳性率最高达36%,β-ME、DMSO分别为33%和25%。结论脐血MSC具有神经干/祖细胞的特性,在一定条件下能向神经元样细胞分化。

Abstract: Objective To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells. Methods The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells(MSCs) were induced by β-mercaptoe-thanol(β-ME), dimethyl sulfoxide(DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase(NSE) and neurofilament(NF) on the treated cells were detected by immunocytochemical method. Results Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by β-ME and 25% of the cells by DMSO also expressed NSE and NF. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.

中图分类号:

R329.24

柳太云¹, 张成¹, 肖露露², 姚晓黎¹, 冯善伟¹, 曾缨¹. 人脐血单个核细胞诱导分化为神经元样细胞[J]. 南方医科大学学报, 2005, 25(02): 152-155.

LIU Tai-yun¹, ZHANG Cheng¹, XIAO Lu-lu², YAO Xiao-li¹, FENG Shan-wei¹, ZENG Ying¹. Induced differentiation of human cord blood monocytes into neuron-like cells in vitro[J]. Journal of Southern Medical University, 2005, 25(02): 152-155.

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