鬼臼毒素硬脂酸固体脂质纳米粒的制备

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (01): 99-101.

鬼臼毒素硬脂酸固体脂质纳米粒的制备

谢方明¹, 曾抗¹, 李国锋², 林中方¹, 孙乐栋¹

1. 南方医科大学南方医院皮肤科, 广东广州510515;
2. 南方医科大学南方医院药学部, 广东广州510515

出版日期:2005-01-20 发布日期:2005-01-20
基金资助:
收稿日期:2004-5-9。
基金项目:广东省科技计划项目(C104034)
作者简介:谢方明(1971- ),男,南方医科大学在读博士研究生,主治医师,电话:020-61641984

Preparation of stearic acid solid lipid nanoparticles containing podophyllotoxin

XIE Fang-ming¹, ZENG Kang¹, LI Guo-feng², LIN Zhong-fang¹, SUN Le-dong¹

1. 南方医科大学南方医院皮肤科, 广东广州510515;
2. 南方医科大学南方医院药学部, 广东广州510515

Online:2005-01-20 Published:2005-01-20

摘要/Abstract

摘要： 目的为提高鬼臼毒素(PPT)制剂的疗效,降低毒副作用,以硬脂酸为载体材料制备PPT固体脂质纳米粒。方法采用改良的微乳技术制备PPT固体脂质纳米粒;用透射电镜考察了纳米粒的形态;用高效液相色谱法测定PPT固体脂质纳米粒的包封率。结果 PPT固体脂质纳米粒基本呈圆球状或椭圆球状,粒径为(56.5±25.8)nm,包封率85.6%。结论 PPT固体脂质纳米粒包封率高,粒径分布较均匀,具有较好的稳定性,是有希望的表皮靶向制剂。

Abstract: Objective To improve the therapeutic efficacy and reduce the adverse effect of podophyllotoxin (PPT) by wrapping it in stearic acid solid lipid nanoparticles. Methods Stearic acid solid lipid nanoparticles containing podophyllotoxin was prepared using modified microemulsion technique, whose morphology was examined by transmission electron microscope. High-performance of liquid chromatography was employed to determine the entrapment efficiency of PPT in the nanoparticles. Results The entrapment efficiency of PPT in the nanoparticles was 85.6% and the mean diameter of the particles was 56.5±25.8 nm. Conclusion The stearic acid solid lipid nanoparticles has high entrapment efficiency for PPT and is homogeneous in size, which can be a promising targeted preparation for epidermal delivery.

中图分类号:

R979.19
R986

谢方明¹, 曾抗¹, 李国锋², 林中方¹, 孙乐栋¹. 鬼臼毒素硬脂酸固体脂质纳米粒的制备[J]. 南方医科大学学报, 2005, 25(01): 99-101.

XIE Fang-ming¹, ZENG Kang¹, LI Guo-feng², LIN Zhong-fang¹, SUN Le-dong¹. Preparation of stearic acid solid lipid nanoparticles containing podophyllotoxin[J]. Journal of Southern Medical University, 2005, 25(01): 99-101.

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