银杏提取物对实验性脊髓空洞症神经元凋亡的影响

南方医科大学学报 ›› 2005, Vol. 25 ›› Issue (01): 83-86.

银杏提取物对实验性脊髓空洞症神经元凋亡的影响

杨国锋¹, 王鲁宁¹, 杨立斌², 张庆俊³

1. 解放军总医院老年神经科, 北京100853;
2. 解放军总医院神经外科, 北京100853;
3. 河北医科大学附属二院神经外科, 河北石家庄050071

出版日期:2005-01-20 发布日期:2005-01-20
基金资助:
收稿日期:2004-5-2。
作者简介:杨国锋(1971- ),男,2004年毕业于解放军军医进修学院,博士,电活:010-66937487,E-mail:yang-guofeng@163.com

Effect of Ginkgo biloba extract on neuronal apoptosis in rabbit with kaolin-induced syringomyelia

YANG Guo-feng¹, WANG Lu-ning¹, YANG Li-bin², ZHANG Qing-jun³

1. 解放军总医院老年神经科, 北京100853;
2. 解放军总医院神经外科, 北京100853;
3. 河北医科大学附属二院神经外科, 河北石家庄050071

Online:2005-01-20 Published:2005-01-20

摘要/Abstract

摘要： 目的评价银杏提取物(GBE)对实验性脊髓空洞症神经元凋亡的影响。方法 30只中国白兔随机分为3组。24只动物经皮枕大池穿刺缓慢抽出0.6ml脑脊液后注入等量25%kaolin,其中12只于当日起给予银杏注射液静脉滴注为银杏组;12只以等量生理盐水静脉滴注为盐水组;6只动物为假手术组作为对照。术后不同时间点灌注处死动物,取脊髓行HE、TUNEL、Bcl-2及Bax观察。结果组织切片对比发现同一时间点银杏组脊髓组织水肿及神经元变性坏死均较盐水组为轻,银杏组脊髓神经元凋亡率、Bax反应阳性细胞率低于盐水组,而Bcl-2反应阳性细胞率高于盐水组。神经元凋亡在术后7d达高峰。结论银杏提取物可改善实验性脊髓空洞kaolin性肉芽肿引起的上颈髓缺血水肿,阻止脊髓神经元凋亡。

Abstract: Objective To evaluate the effect of Ginkgo biloba extract (GBE) on neuronal apoptosis in rabbits with kaolin-induced syringomyelia. Methods Twenty-four of 30 Chinese white rabbits were subjected to injection of 25% kaolin mixed with equal volume (0.6 ml) of cerebrospinal fluid drawn from the cisterna magna under ketamine anesthesia. Twelve of these 24 rabbits then received intravenous injection of 5 ml of GBE (5 ml/days for 14 days, GBE treatment group) while the other 12 were treated with the same amount of saline administered in similar manner (saline group). The 6 rabbits without kaolin treatment received a sham operation to serve as the control group. At different time points after the operation, the rabbits were killed and the spinal cord samples examined by immunohistochemistry. Results Histologically, ischemia and edema in the cervical cord of rabbits in GBE treatment group were less severe than those in saline group. TUNEL-positive and bax-positive neurons were less numerous in GBE treatment group than in saline group, and the former group showed more Bcl-2-positive neurons. The number of apoptotic neurons reached the peak level on day 7 after kaolin injection. Conclusion GBE can ameliorate kaolin-induced hydrocephalus in the upper cervical cord and inhibit kaolin-induced neuron apoptosis.

中图分类号:

杨国锋¹, 王鲁宁¹, 杨立斌², 张庆俊³. 银杏提取物对实验性脊髓空洞症神经元凋亡的影响[J]. 南方医科大学学报, 2005, 25(01): 83-86.

YANG Guo-feng¹, WANG Lu-ning¹, YANG Li-bin², ZHANG Qing-jun³. Effect of Ginkgo biloba extract on neuronal apoptosis in rabbit with kaolin-induced syringomyelia[J]. Journal of Southern Medical University, 2005, 25(01): 83-86.

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