南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (11): 1283-1286.

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山东省家猪感染肾综合征出血热病毒流行病学调查和实验研究

杨占清1, 俞守义1, 聂军1, 陈清1, 李志峰1, 刘运喜2, 张景兰3, 徐建江2, 于晓敏2, 步秀萍2, 苏静静2   

  1. 1. 第一军医大学流行病学教研室, 广东, 广州, 510515;
    2. 济南军区联勤部军事医学研究所, 山东, 济南250014;
    3. 南京军区联勤部军事医学研究所, 江苏, 南京, 210002
  • 出版日期:2004-11-20 发布日期:2004-11-20
  • 基金资助:
    收稿日期:2004-5-8。
    作者简介:杨占清(1951- ),男,研究员,1999年被批准亨受国务院特殊津贴,电话:020-61648311

Prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs:an epidemiological investigation in Shandong province

YANG Zhan-qing1, YU Shou-yi1, NIE Jun1, CHEN Qing1, LI Zhi-feng1, LIU Yun-xi2, ZHANG Jin-lan3, XU Jian-jiang2, YU Xiao-ming2, BU Xiu-ping2, SU Jing-jing2   

  1. 1. 第一军医大学流行病学教研室, 广东, 广州, 510515;
    2. 济南军区联勤部军事医学研究所, 山东, 济南250014;
    3. 南京军区联勤部军事医学研究所, 江苏, 南京, 210002
  • Online:2004-11-20 Published:2004-11-20

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摘要： 目的 了解家猪在肾综合征出血热(HFRS)疫区的流行病学意义,探讨家猪与HFRS发病的关系。方法 以HFRS病毒(HFRSV)抗原和抗体为指标,采用反向间接血凝法(RPHA),血凝抑制法(RPHI),酶标葡萄球菌A蛋白(HPR-SPA),间接免疫荧光法(IFA)和PCR反应对山东部分地区家猪自然感染HFRSV的情况进行检测。结果 家猪心、肝、脾、肺、肾等脏器及血、尿、粪和猪圈内污物检出HFRSV抗原与抗体,脏器抗原阳性率为3.33%~5.00%。而血、尿、粪和圈内的污染物抗原阳性率分别为3.67%、7.04%、2.51%和5.56%,血清抗体阳性率为1.96%,并从HFRSV抗原阳性的多种标本分离出病毒。人工感染后病毒可随血液波及多个脏器,二次感染乳鼠脑可检出HFRSV抗原。二次组织病理学检查发现肺、肝、肾等脏器有一过性病理改变;HFRSV可在猪体内增殖,并经多途经排出体外。结论 家猪可能是HFRSV的扩散宿主动物。

Abstract: Objective To investigate the epidemiological significance of hemorrhagic fever with renal syndrome virus (HFRSV) infection in domestic pigs in Shandong province, and study the role of domestic pigs in the prevalence of HFRS.Methods Epidemiological investigation was performed in 4 cities of Shandong province. Reversed passive hemagglutination assay (RPHA), reversed passive hemagglutination inhibition (RPHI), HPR-SPA, immunofluorescent antibody (IFA), and reverse transcriptional PCR (RT-PCR) were used to detect antigen and antibody of HFRSV.Results HFRSV antigen and antibody were detected in the heart, liver, lung, spleen, kidney, blood, urine, and stool of domestic pigs as well as in the sewage of the pigpen facilities. The positivity rate of HFRSV antigen ranged from 3.33% to 5.00% in the organ of pigs, and HFRSV positivity rate in the blood, urine, stool, and sewage was 3.67%, 7.04%, 2.51%, and 5.56%, respectively, with a total serum antibody positivity rate of 1.96%. The virus was isolated from the HFRSV antigen-positive samples, and could infect many organs after artificial infection of the pigs. HFRSV antigen can be detected in suckling rat brain and histopa- thological exanimation suggested transient pathological changes in such organs as the liver, lung and kidney. HFRSV may proliferate in pigs and was discharged through multiple routes.Conclusion Domestic pigs can be the host of HFRSV for the viral transmission.

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