血清蛋白质组学研究中白蛋白和IgG去除方法的建立与评价

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (11): 1233-1235.

血清蛋白质组学研究中白蛋白和IgG去除方法的建立与评价

李雪华¹, 李欣², 徐守军³, 丁彦青¹

1. 南方医科大学, 南方医院病理科, 广东, 广州, 510515;
2. 南方医科大学, 肿瘤研究所, 广东, 广州, 510515;
3. 军事医学科学院, 北京, 100850

出版日期:2004-11-20 发布日期:2004-11-20
基金资助:
收稿日期:2004-4-29。
基金项目:广东省科技重大专项(2003A308401);国家重点基础研究发展规划项目(973)(2001CB510208)
作者简介:李雪华(1974- ),男,讲师,硕士,电话:020-61640114-89100,E-mail:snow110@fimmu.com通讯作者:丁彦青,电话020-61642148,E-mail:dyq@fimmu.com.;李欣,电话020-61648226,E-mail:lixin@fimmu.com
通讯作者:丁彦青,电话:020-61642148,E-mail:dyq@fimmu.com;李欣,电话:020-61648226,E-mail:lixin@fimmu.com

Establishment and evaluation of the method for removing albumin and IgG in serum proteomics

LI Xue-hua¹, LI Xin², XU Shou-jun³, DING Yan-qing¹

1. 南方医科大学, 南方医院病理科, 广东, 广州, 510515;
2. 南方医科大学, 肿瘤研究所, 广东, 广州, 510515;
3. 军事医学科学院, 北京, 100850

Online:2004-11-20 Published:2004-11-20

摘要/Abstract

摘要： 目的建立和评价血清蛋白质组学研究中白蛋白和IgG的去除方法。方法应用Micro Bio-Spin column过滤纯化血清蛋白,去除白蛋白和IgG,利用双向凝胶电泳(2-DE)分析评价血清蛋白过滤效果。结果建立了血清白蛋白和IgG去除方法;过滤后大部分血清白蛋白和IgG能够去除,2-DE图谱中低丰度蛋白质分辨率增加。结论本研究建立的血清蛋白质纯化技术,可有效应用于疾病的血清蛋白质组学研究。

Abstract: Objective To establish and evaluate the method for removing albumin and IgG in serum proteomics.Methods Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE).Results The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map.Conclusion The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.

中图分类号:

R-33

李雪华¹, 李欣², 徐守军³, 丁彦青¹. 血清蛋白质组学研究中白蛋白和IgG去除方法的建立与评价[J]. 南方医科大学学报, 2004, 24(11): 1233-1235.

LI Xue-hua¹, LI Xin², XU Shou-jun³, DING Yan-qing¹. Establishment and evaluation of the method for removing albumin and IgG in serum proteomics[J]. Journal of Southern Medical University, 2004, 24(11): 1233-1235.

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