斑蝥素对G1/S期肾小管上皮细胞ATP的调节

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (10): 1192-1193,1196.

斑蝥素对G₁/S期肾小管上皮细胞ATP的调节

沈青¹, 姚裕家², 李如英¹, 丁翠玲¹, 侯立朝³, 孙绪德³

1. 第四军医大学西京医院儿科, 陕西, 西安, 710032;
2. 四川大学华西二院儿科, 四川, 成都, 610041;
3. 第四军医大学西京医院麻醉科, 陕西, 西安, 710032

出版日期:2004-10-20 发布日期:2004-10-20
基金资助:
收稿日期:2004-2-18。
作者简介:沈青(1963- ),男,医学博士,副主任医师,电话:029-3375399,E-mail:mailsq@fmmu.edu.cn

Cantharidin regulates intracellular ATP in G₁/S-phase renal tubule epithelial cells

SHEN Qing¹, YAO Yu-jia², LI Ru-ying¹, DING Cui-ling¹, HOU Li-chao³, SUN Xu-de³

1. 第四军医大学西京医院儿科, 陕西, 西安, 710032;
2. 四川大学华西二院儿科, 四川, 成都, 610041;
3. 第四军医大学西京医院麻醉科, 陕西, 西安, 710032

Online:2004-10-20 Published:2004-10-20

摘要/Abstract

摘要： 目的通过斑蝥素对G₁/S期肾小管上皮细胞(RTECs)低氧损伤后细胞ATP代谢影响的研究,探讨斑蝥素稳定肌动蛋白微丝骨架系统的机制。方法将新生猪G₁/S界面RTECs分为3组:实验组(氰化钠+斑蝥素)、缺氧组(氰化钠)和空白对照组,应用反相高效液相比较缺氧后各组细胞内ATP含量的变化。结果实验组细胞内ATP含量[(14.50±0.26)mmol/g蛋白]明显高于缺氧组[(4.25±0.11)mmol/g蛋白]和空白对照组[(8.58±0.13)mmol/g蛋白](P<0.01)。结论斑蝥素稳定肌动蛋白微丝骨架系统可能与抗RTECs低氧损伤后细胞能量代谢紊乱相关。

Abstract: Objectives To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G₁/S-phase renal tubule epithelial cells (RTECs).Methods G₁-phase RTECs were divided into cantharidin-treated group,exposed to sodium cyanide (CN) and cantharidin,hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography. Results The concentration of CN exposure for 1 h,ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50±0.26 mmol/g protein,4.25±0.11 mmol/g protein,8.58±0.13 mmol/g protein,respectively,P<0.01).Conclusion Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.

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沈青¹, 姚裕家², 李如英¹, 丁翠玲¹, 侯立朝³, 孙绪德³. 斑蝥素对G₁/S期肾小管上皮细胞ATP的调节[J]. 南方医科大学学报, 2004, 24(10): 1192-1193,1196.

SHEN Qing¹, YAO Yu-jia², LI Ru-ying¹, DING Cui-ling¹, HOU Li-chao³, SUN Xu-de³. Cantharidin regulates intracellular ATP in G₁/S-phase renal tubule epithelial cells[J]. Journal of Southern Medical University, 2004, 24(10): 1192-1193,1196.

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斑蝥素对G₁/S期肾小管上皮细胞ATP的调节

Cantharidin regulates intracellular ATP in G₁/S-phase renal tubule epithelial cells

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