脑源性神经营养因子调控人黄素化颗粒细胞甾体激素合成

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (10): 1174-1176.

脑源性神经营养因子调控人黄素化颗粒细胞甾体激素合成

陈炜, 陈士岭, 邢福祺

南方医科大学南方医院妇产科生殖医学中心, 广东, 广州, 510515

出版日期:2004-10-20 发布日期:2004-10-20
基金资助:
收稿日期:2004-6-7。
作者简介:陈炜(1975- ),男,现工作单位为南方医科大学珠江医院妇产科,E-mail:zschenwei75@yahoo.com.cn

Brain-derived neurotrophin factor inhibits steroid biosynthesis by human granulosa-lutein cells

CHEN Wei, CHEN Shi-ling, XING Fu-qi

南方医科大学南方医院妇产科生殖医学中心, 广东, 广州, 510515

Online:2004-10-20 Published:2004-10-20

摘要/Abstract

摘要： 目的探讨脑源性神经营养因子(BDNF)对人黄素化颗粒细胞分泌雌激素和孕激素及甾体生成急性调控蛋白(STAR)的影响。方法黄素化壁层颗粒细胞来自进行体外受精-胚胎移植的26例患者,年龄26~30岁,均为输卵管因素和男性因素不孕者。利用原代培养细胞技术培养人黄素化颗粒细胞,分别加入不同浓度的BDNF,收集细胞培养上清液,检测雌二醇和孕酮浓度。用RT-PCR检测黄素化颗粒细胞中STARmRNA表达的变化。结果 BDNF可抑制人黄素化壁层颗粒细胞分泌孕激素,呈浓度依赖性,但BDNF浓度超过50ng/ml时抑制作用不再增强。BDNF对人黄素化壁层颗粒细胞合成雌二醇的浓度无明显影响。BDNF可降低STARmRNA表达,呈浓度依赖性。结论 BDNF可调节人黄素化卵巢颗粒细胞合成孕酮,是重要的卵巢内调节因子。BDNF可能通过抑制STARmRNA的表达以减少孕酮的生成并参与黄体的退化过程。

Abstract: Objective To study the effect of brain-derived neurotrophin factor (BDNF) on the synthesis of estradiol and progesterone in human granulose-lutein cells (HGLCs) and the expression of steroidogenic acute regulatory factor (STAR) mRNA.Methods HGLCs were obtained from infertile women undergoing ovulation induction for fertilization-embryo transfer (IVF-ET) for male or tubal factors. HGLCs were cultured in serum-free media 199 for 24 h and treated by BDNF at 25,50 and 100 ng/ml. Radio immunoassay (RIA) was used to examine the concentration of estradiol and progesterone,and reverse transcriptional PCR (RT-PCR) employed to detect the expression of STAR mRNA after treatment with BDNF at the concentrations of 25,50 and 100 ng/ml. Results BDNF significantly inhibited the production of progesterone (P₄) in the culture media of HGLCs in a dose-dependent manner. BDNF did not change the level of 17β-estradiol (E₂),but decreased the expression of STAR mRNA dose-dependently.Conclusions BDNF can inhibit the synthesis of P4 in HGLCs and regulate ovarian steroidogenesis. BDNF may inhibit HGLCs from producing P4 by decreasing the transcription level of STAR gene in human ovary,and plays an important role in luteal regression.

中图分类号:

R339.22

陈炜, 陈士岭, 邢福祺. 脑源性神经营养因子调控人黄素化颗粒细胞甾体激素合成[J]. 南方医科大学学报, 2004, 24(10): 1174-1176.

CHEN Wei, CHEN Shi-ling, XING Fu-qi. Brain-derived neurotrophin factor inhibits steroid biosynthesis by human granulosa-lutein cells[J]. Journal of Southern Medical University, 2004, 24(10): 1174-1176.

参考文献

[1] Seifer DB,Feng B,Shelden RM,et al.Brain-derived neurotrophic factor:a novel human ovarian follicular protein [J].J Clin Endocrinol Metab,2002,87(2):655-9.
[2] Seifer DB,Lambert-Messerlian G.Ovarian brain-derived neurotrophic factor is present in follicular fluid from normally cycling women [J].Fertil Steril,2003,79(2):451-2.
[3] 付娟,邢福祺,陈士岭.酪氨酸蛋白激酶受体B在人卵泡中的表达及意义[J].第一军医大学学报,2003,23(2):162-4.Fu J,Xing FQ,Chen SL.Expression oftyrosine kinase receptor B in human follicle [J].J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao,2003,23(2):162-4.
[4] Feng B,Chen S.Effect of gonadotropins on brain-derived neurotrophic factor secretion by human follicular cumulus cells [J].Fertil Steril,2003,80(3):658-9.
[5] Jensen T,Johnson AL.Expression and function of brain-derived neu rotrophin factor and its receptor,TrkB,in ovarian follicles from the domestic hen (Gallus gallus domesticus)[J].J Exp Biol,2001,204(12):2087-95.
[6] Lavoie HA,Bnoit AM,Garmey JC,et al.Coordinate developmental expression of gene regulating stero economy and cholesterol side-chain cleavage in the porcine ovary[J].Biol Reprod,1997,57(2):402-7.
[7] Gregory CW,Dephilp RM.Detection of steroidogenic acute regulatory factor (STAR) in mitochondria of cultured rat Sertoli-cells incubated with follicle-stimulating hormone [J].Biol Reprod,1998,58(2):470-4.
[8] Kerban A,Boerboom D,Sirois J,et al.Human chorionic gonadortopin induce an inverse regulation of steroidogenic acute regulatory factor messenger ribonucleic acid in the theca intema and granulosa cells of eqine preovulatory follicles [J].Endocrinology,1999,140(2):667-74.
[9] Chen YJ,Feng Q,Liu YX,et al.Expression of steroidogenic acute regulatory factor and luteinizing hormone receptor and their regulation by tumor necrosis factorin the cat corpora lutea[J].Biol Reprod,1999,60(2):419-27.
[10] Jakimiuk AJ,Weitsman SR,Navab A,et al.Luteinizing hormone receptor,steroidogenesis acute regulatory protein,and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries [J].J Clin Endocrinol Metab,2001,86(3):1318-23.