新生小牛血清对人胎脑海马组织神经干细胞分化的影响

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (10): 1164-1166,1170.

新生小牛血清对人胎脑海马组织神经干细胞分化的影响

尹晓娟, 封志纯, 杜江

南方医科大学珠江医院儿科, 广东, 广州, 510282

出版日期:2004-10-20 发布日期:2004-10-20
基金资助:
收稿日期:2004-2-16。
作者简介:尹晓娟(1966- ),女,在读博士研究生,现为第三军医大学西南医院儿科主治医师,主要从事新生儿疾病、遗传性疾病及胚胎神经发育方面的研究
通讯作者:封志纯,教授,电话:020-61643369

Effects of neonatal calf serum on differentiation of human fetal neural stem cells in the hippocampus

YIN Xiao-juan, FENG Zhi-chun, DU Jiang

南方医科大学珠江医院儿科, 广东, 广州, 510282

Online:2004-10-20 Published:2004-10-20

摘要/Abstract

摘要： 目的探讨新生小牛血清对人胎脑海马组织神经干细胞分化的影响。方法采用细胞培养和免疫细胞组化技术,观察不同浓度新生小牛血清对人胎脑海马组织神经干细胞分化的影响。结果新生小牛血清能诱导人神经干细胞分化为神经细胞及神经胶质细胞,在无血清培养基与含不同浓度新生小牛血清培养基中培养的神经干细胞分化为神经细胞和神经胶质细胞的数目在发生变化,并且随着新生小牛血清浓度的增加,神经干细胞分化为神经细胞的数量在减少,分化为神经胶质细胞的数量增加。结论新生小牛血清影响人神经干细胞分化为神经细胞和神经胶质细胞的比例,并与新生小牛血清的浓度有一定的关系。

Abstract: Objective To investigate the effects of neonatal calf serum on the differentiation of human neural stem cells in the hippocampus.Methods The effects of various concentrations of neonatal calf serum on the differentiation of human fetal neural stem cells in the hippocampus were observed in cell culture experiment and immunocytochemical staining. Results Neonatal calf serum efficiently induced the differentiation of human fetal neural stem cells into neurons and astrocytes,whose amount varied between serum-free cell culture and the culture with neonatal calf serum of different concentrations. As the concentration of neonatal calf serum increased,the differentiation of human fetal neural stem cells shifted toward astrocytes,while the differentiation into neurons was decreased.Conclusion Neonatal calf serum causes alteration of the ratio between neurons and glial cells differentiated from human neural stem cells in the hippocampus.

中图分类号:

尹晓娟, 封志纯, 杜江. 新生小牛血清对人胎脑海马组织神经干细胞分化的影响[J]. 南方医科大学学报, 2004, 24(10): 1164-1166,1170.

YIN Xiao-juan, FENG Zhi-chun, DU Jiang. Effects of neonatal calf serum on differentiation of human fetal neural stem cells in the hippocampus[J]. Journal of Southern Medical University, 2004, 24(10): 1164-1166,1170.

参考文献

[1] 梁鹏,李庆国,刘恩重.人胚胎皮层神经干细胞的分离及鉴定[J].中华医学杂志,2001,80(21):1324-6.Liang P,Li QG,Liu EZ.Isolation and identification of neural precursor cells from human embryonic cortical tissue[J].Natl Med J Chin,2001,80(21):1324-6.
[2] 余小强,阮怀珍,蔡文琴,等.PD大鼠纹状体提取液诱导中脑神经干细胞分化为多巴胺能神经元的研究[J].第三军医大学学报,2004,26(1):18-21.Yu XQ,Ruan HZ,Cai WQ,et al.Striatal extracts of rats induce differentiation of mesencephalic neural stem cells into dopaminergic neurons[J].J Third Mil Med Univ,2004,26(1):18-21.
[3] 王怀忠.神经干细胞在中枢神经系统损伤修复中的应用前景[J].中国康复理论与实践,2004,10(1):1.Wang HZ.Applied prospect of neural stem cells in repaired central nervous system[J].Chin J Rehabil Theory Pract,2004,10(1):1.
[4] Nishino H,Hida H,Takei N,et al.Mesencephalic neural stem(progenitor) cells develop to dopaminergic neurons more strongly in dopamine depleted striatum than in intact striatum [J].Exp Neurol,2000,164(1):209-14.
[5] Flax J,Aurora S.Engraftable human stem cells respond to cues,replace,and express foreign genes [J].Nat Biotech,1998,16(2):1033-99.
[6] Parmentier ML,Woods D,Greig S,et al.Rapsynoid/partner of inscuteable controls asymmetric division of larval neuroblasts in Drosophila[J].J Neurosci,2000,20(84):1-5.
[7] Arsenijevic Y,Weiss S,Schneider B,et al.Insulin-like growth facto-I is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2 [J].J Neurosci,2001,21 ( 18):7194-202.
[8] Brannen CL,Sugaya SO.In vitro differentiation of multipotent human neural progenitors in serum-free medium[J].Neuroreport,2000,11(5):1123-8.
[9] 尹晓娟,封志纯,杜江.分离培养不同胚龄人神经干细胞的方法比较[J].中国临床康复,2004,8(9):1726-7.Yin XJ,Feng ZC,Du J.Comparison of the methods on isolation culture and identification of human neural stem cells from different embryonic brains[J].Chin J Clin Rehabil,2004,8(9):1726-7.
[10] 尹晓娟,封志纯.神经干细胞应用进展[J].中国临床康复,2004,8(4):714-5.Yin XJ,Feng ZC.Applied prospect on neural stem cells[J].Chin J Clin Rehabil,2004,8(4):714-5.
[11] 尹晓娟,封志纯.神经干细胞增殖和分化的影响因素[J].中国儿童保健,2004,12(3):242-3.Yin XJ,Feng ZC.Effective factors about proliferation and differentiation of neural stem cells[J].Chin J Child Health Care,2004,12(3):242-3.
[12] 李巍,蔡文琴,吴康,等.人胚胎大脑皮层神经干细胞的分离培养[J].解剖学报,2002,33(3):241-4.Li W,Cai WQ,Wu K,et al.Characterization of neural stem cells isolated from human fetal cerebral cortex in vitro[J].Acta Anat Sin,2002,33(3):241-4.
[13] 姜传涛,杨秋慧,苏玉金,等.胎儿脑组织体外保存获取神经干细胞的时限[J].解剖学报,2002,33(3):449-52Jiang CT,Yang QH,Su YJ,et al.The time limitation of incubation for isolating neural stem cells from human fetal brain [J].Acta Anat Sin,2002,33(3):449-52.
[14] 陆华,蒋云召,苗宗宁,等.人胚脑皮层神经干细胞的分离培养及鉴定[J].中国微循环,2002,6(1):9-10.Lu H,Jiang YZ,Miao ZN,et al.Isolation culture and identification of neural stem cells from human embryonic cerebral cortex [J].J Chin Microcirc,2002,6(1):9-10.
[15] Santa-Olalla J.Basic fibroblast growth factor promotes epidermal growth factor responsiveness and survival of mesencephalic neural precuror cells[J].J Neurobiol,1999,40(1):14-27.
[16] Calli R.Regulation of neuronal differentiation in human CNS stem cell progeny by leukemia inhibitory factor [J].Dev Neurosci,2000,22(3):86-95.