南方医科大学学报

南方医科大学学报 ›› 2004, Vol. 24 ›› Issue (10): 1156-1159.

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线栓法大鼠缺血/再灌注脑损伤模型的改良

蒋海山, 陆兵勋, 姬仲, 王立新, 张素娟   

  1. 南方医科大学南方医院神经内科, 广东, 广州, 510515
  • 出版日期:2004-10-20 发布日期:2004-10-20
  • 基金资助:
    收稿日期:2004-3-19。
    作者简介:蒋海山(1978- ),男,硕士,助教,电话:020-61641965,E-mail:icdi_dr@fimmu.com

Surgical procedure improvement to produce rat ischemia-reperfusion injury model with intraluminal suture

JIANG Hai-shan, LU Bing-xun, JI Zhong, WANG Li-xin, ZHANG Su-juan   

  1. 南方医科大学南方医院神经内科, 广东, 广州, 510515
  • Online:2004-10-20 Published:2004-10-20

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摘要： 目的 研究一种线栓法大鼠缺血/再灌注脑损伤模型的改良术式。方法 结扎颈总动脉(CCA),不结扎颈外动脉和翼腭动脉,用头皮针于CCA结扎的远端穿刺导入3-0线栓造成缺血,通过拔出线栓形成再灌注。结果 栓线长度(20.0±1.8)mm,造模成功率近70%,造模动物临床表现及病理表现典型。结论 本术式简便,术者不需具备显微手术操作技巧,造模成功率较高。

Abstract: Objective To describe a modified surgical approach to produce rat ischemia-reperfusion injury model with intraluminal suture.Methods After exposure and ligation of the common carotid artery (CCA),the left external carotid artery and pterygopalatine artery were opened in which a 3-0 nylon suture was introduced intraluminally from the distal end of the ligature of the CCA using a scalp needle. Reperfusion injury was induced by withdrawal of the suture. Results The length of the suture was about 20.0±1.8 mm and the success rate of model establishment was nearly 70%. The rats developed typical symptoms and pathological manifestations after the surgery.Conclusion This modified surgical approach is simple for model establishment and does not require special microsurgical skills to ensure the high success rate.

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